ABSTRACT
Objective To develop a simple,rapid and accurate analysis method for determination of L-corydalmine(L-CDL) in rat plasma. Methods The plasma sample was determined with propranolol as the internal standard(IS),the separation was ac?complished in a Capcell PAK C18(2.1 mm×750 mm,3μm)column,and the mobile phase consisted of water( including 0.1%formic acid and 5 mmol/L ammonium formate)and acetonitrile(including 0.1%formic acid)at a flow rate of 0.25 ml/min. The mass spec?trometer consisted of an ESI interface operating at positive ionization mode and the detection was performed using multiple reaction monitoring(MRM)at the transitions of m/z 342?192.1 for L-CDL and m/z 260?116.2 for propranolol. Method validation included the evaluation of the linearity range,lower LOQ,within-run and between-run precision,accuracy and stability,matrix effect and extrac?tion recovery. Results The calibration curve was linear across the concentration range of 1-5000 ng/ml for L-CDL with a lower LOQ of 1 ng/ml. The within-run and between-run precision(RSD%)was in the range 0.1%-10%. The extraction recovery was in 93.5%-102.8%and the matrix effect for three QC was 108.3%-112.5%. L-CDL reached the peak concentration at 0.5 h after dosing. The main pharmacokinetic parameters of rats after igl administration were as follows:T1/2:(7.04 ± 3.93)h,Cmax:(557.8 ± 330.1)ng/ml,AUC0~24:(2408±630)h·ng/ml. Conclusion A simple,rapid,accurate,high sensitivity and repeatability method has been successfully devel?oped,which can analyze the concentration of L-CDL in rat plasma. The method can be used for the investigation of pharmacokinetics of L-CDL in rats.
ABSTRACT
Aim To study the antipsychotic effects of l-Scoulerine ( l-SLR) . Methods NMDAreceptorantag-onist MK-801 was used to induce the positive and neg-ative symptoms of schizophrenia and cognitive impair-ment in animal models. The effects of l-SLR were eval-uated on schizophrenia induced by MK-801 and on ex-trapyramidal system. Results l-SLR (10,15 mg · kg - 1 , ip) could suppress pre-pulse inhibition damage in rats induced by MK-801 (0. 3 mg·kg - 1 , ip); l-SLR(30 mg·kg - 1 , ip) could inhibit climbing behav-iors in mice induced by apomorphine, which suggested that l-SLR had significant inhibiting effects on the posi-tive symptoms of schizophrenia by MK-801 and apo-morphine. l-SLR could also induce social contact inhi-bition and cognitive impairment induced by MK-801 (0. 2 mg · kg - 1 , ip), which proposed that l-SLR could improve the negative symptoms and cognitive im-pairment by MK-801. Catalepsy in mice could be caused by the treatment dose of haloperidol (0. 8 mg· kg - 1 , ip), not by that of l-SLR(30 mg·kg - 1 , ip). Conclusion I-SLR has significant effects on the posi-tive and negative symptoms of schizophrenia and cogni-tive impairment and, the effect of l-SLR under effective dose on extrapyramidal system is obviously much less than that of haloperidol and l-SPD.