ABSTRACT
Objective To understand the situation of seroepidemiology of Toxoplasma gondii infection among employees in a pork food processing enterprise in Dongtai City. Methods Totally 200 employees from a pork food processing enterprise in Dongtai City were selected as the research objects. The venous blood of the selected population was extracted and the IgG antibody against T. gondii in serum was detected by ELISA, and the results were analyzed. Results Among the 200 employees, 36 cases were positive in IgG antibody detection, the positive rate was 18.0%. The positive rate of IgG antibody among workers with different length of service was significantly different (χ2 = 9.813, P < 0.05). With the increase of working age, the positive rate of IgG antibody gradually increased. The positive rate of IgG antibody to T. gondii was significantly higher in the population who had frequent contact with animals. Conclusions The positive rate of IgG antibody against T. gondii in employees in pork food processing enterprises is high. Therefore, the detection of antibodies against T. gondii and health education should be strengthened in this population.
ABSTRACT
Objective To understand the situation of seroepidemiology of Toxoplasma gondii infection among employees in a pork food processing enterprise in Dongtai City. Methods Totally 200 employees from a pork food processing enterprise in Dongtai City were selected as the research objects. The venous blood of the selected population was extracted and the IgG antibody against T. gondii in serum was detected by ELISA, and the results were analyzed. Results Among the 200 employees, 36 cases were positive in IgG antibody detection, the positive rate was 18.0%. The positive rate of IgG antibody among workers with different length of service was significantly different (χ2 = 9.813, P < 0.05). With the increase of working age, the positive rate of IgG antibody gradually increased. The positive rate of IgG antibody to T. gondii was significantly higher in the population who had frequent contact with animals. Conclusions The positive rate of IgG antibody against T. gondii in employees in pork food processing enterprises is high. Therefore, the detection of antibodies against T. gondii and health education should be strengthened in this population.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether Hantavirus (HV) and Orientia tsutsugamushi ( OT) can naturally infect and coexist in their host and role.</p><p><b>METHODS</b>By field epidemiological study, Leptotrombidium scutellare (3829) was collected and separated from mice(166) in epidemic areas. The cells of mites separated from their host and role were cultured. PCR was used to detect HV-RNA and OT-DNA in the cell culture.</p><p><b>RESULTS</b>In 105 Apodemus agrarius, 3 HV-RNA positive, 2 OT-DNA positive and 2 coinfection with HV and OT were detected;in 41 Brown rattus, 2 HV-RNA positive, 1 OT-DNA positive and 1 co-infection with HV and OT were detected. From 15 mites co-infected with HV and OT, 2 strains of HV pathogen, 2 strains of OT pathogen were separated and 1 HV and OT pathogen in the same mite were separate.</p><p><b>CONCLUSION</b>The study demonstrates that co-infection of HV and OT did simultaneously exist in wild Leptotrombidium scutellare. This theory has some significance to the epidemic and precaution of HV and OT.</p>