ABSTRACT
Objective To provide experimental basis for the identification of Vitex negundo var. cannabifolia fruits and V. negundo fruits by comparative study. Methods The original plant identification, morphological identification, microscopic identification, TLC and HPLC identification were adopted to distinguish the two kinds of materials. Results It is difficult to tell the difference of these two medicinal fruits in term of original plant identification and morphological identification to their similarity, while microscopic identification, TLC and HPLC identification revealed that the characteristics of sclereid could be used to distinguish these two fruits. Conclusion Microscopic identification on the sclereid characteristics of fruits powder, combined with TLC and HPLC identification can be the feasible method that used to distinguish V. negundo fruits and V. negundo var. cannabifolia fruits.
ABSTRACT
A pairs of primers were designed according to the fusion protein(F)gene sequences of canine distemper virus(CDV)in GenBank.A 369 bp fragment aimed signal peptide fragment of F gene was amplified.The PCR products from viscera samples,blood,urine of fur animals including foxes,minks and raccoon dogs,which collected in the years 2005-2007,were cloned to pMD18-T Vector and sequenced.We obtained 13 positive signal peptide fragments from wild-type strains.The results indicated there was obviously genetic diversity between the wild-type strains and CDV3 and other vaccine strains.The homology with CDV3 is 80.7%-83.2%in nucleotide,and 64.8%-71.3%in amino acid.The analysis for the hydrophobic regions indicated the function of signal peptide fragment may be changed.This study can offer aca- demic data to research of CDV genetic variation and epidemiology.