ABSTRACT
<p><b>OBJECTIVE</b>To investigate the association of expression of c-kit (marker of interstitial cells of Cajal, ICC) in colon with slow transit constipation (STC) in rats.</p><p><b>METHODS</b>Slow transit constipation (STC) rat model was induced by intragastric administration of compound diphenoxylate. Western blotting was used to measure the expression of c-kit in colon of STC rats (model group) and normal rats (control group). Gray scale ratio of c-kit to β-actin was used as the relative quantity of c-kit.</p><p><b>RESULTS</b>Fecal quantity per day of STC group was (1.3±0.7) g/100 g, significantly lower than that in normal rats [(1.6±0.9) g/100 g, t=10.798, P<0.05]. In model rats, the time of discharge of the first black fecal was (461.6±150.8) min, significantly longer than that in normal rats [(351.3±119.9) min, t=2.291, P<0.05]. Western blotting revealed that the average values of gray scale ratio of c-kit in proximal colon were 0.277±0.077 and 0.576±0.081 (t=10.719, P<0.05), in distal colon were 0.280±0.075 and 0.571±0.079 (t=10.700, P<0.05) in model group and control group respectively.</p><p><b>CONCLUSION</b>Down-regulation of c-kit expression in proximal colon and distal colon is associated to the pathogenesis of slow transit constipation in rats.</p>
Subject(s)
Animals , Female , Male , Rats , Chronic Disease , Colon , Metabolism , Pathology , Constipation , Metabolism , Pathology , Disease Models, Animal , Interstitial Cells of Cajal , Pathology , Proto-Oncogene Proteins c-kit , Metabolism , Rats, Sprague-DawleyABSTRACT
<p><b>OBJECTIVE</b>To study the characteristics of thyroglossal tract cyst epidemiology and summarize how to improve the operation therapeutic effect.</p><p><b>METHODS</b>The clinic appearance, the pathological characteristics and the therapeutic effect of the 60 thyroglossal tract cyst cases were analyzed.</p><p><b>RESULTS</b>Thyroglossal tract cyst mostly occurred in children and teenagers. The incidence of female patients was more than that of male patients. Most of thyroglossal tract cyst located in the middle line of the neck and were explored tubular-objects and fibroropes. The histopathological feature was variform.</p><p><b>CONCLUSION</b>The clinical appearance and pathology characteristic of thyroglossal tract cyst are variform. Thyroglossal tract cyst may be recurred easily if it is not operated well.</p>