ABSTRACT
Objective There are few reports on the relationship between lncRNA cancer susceptibility candidate gene 2 (CASC2) and NF-κB signaling pathway in thyroid papillary carcinoma cells at home and abroad. This study aimed to investigate the effect of lncRNA CASC2a on the proliferation and migration of TPC-1 via NF-κB signaling pathway. Methods TPC-1 was selected and constructed into lncRNA CASC2a overexpression plasmids which were divided into plasmid group [transfection with overexpressed plasmid pcDNA3.1(+)-CASC2a], empty group [transfection with equal amount of pcDNA3.1 (+) empty plasmid], blank group (without any processing). The effect of overexpressed lncRNA CASC2a on the expression of CASC2a in each group of TPC-1 cells was examined. The cells of transfected plasmid group were randomly divided into transfection group [transfection with only overexpressed plasmid pcDNA3.1(+)-CASC2a], transfection + PMA group [transfection with overexpressed plasmid pcDNA3.1(+)- CASC2a + propylene glycol methyl ether acetate (PMA) stimulation], control group (without any processing), PMA group (only plus PMA stimulation). The effects of lncRNA CASC2a on cell proliferation and migration were verified by CCK-8 and cell scratch assays at 24, 48, 72 and 96 h. Western blot was used to detect the expression of p105/p50 and p65 in NF-κB signaling pathway. The expression of NF-κB signaling pathway-related antibody proteins p105/p50 and p65 was observed by NF-κB signaling pathway agonist PMA(propylene glycol methyl ether acetate). Results After transfection with overexpressed plasmid pcDNA3.1(+)-CASC2a, the expression of CASC2a in plasmid group was significantly higher than that in empty group (P<0.05). The cell proliferation ability (0.191±0.005) was significantly lower in transfection+PMA group than that in transfection group (0.217±0.013), PMA group (0.247±0.009), and control group (0.260±0.004), and the difference was statistically significant ( P<0.01), while the cell proliferation ability in transfection group was significantly lower than those in PMA group and control group (P<0.01). The cell migration ability of transfected + PMA group [(0.208±0.109)%] was lower than that of transfection group, PMA group, and control group [(1.775±0.061)%, (1.622±0.519)%, (2.927±0.136)%], while the cell migration ability of transfection group and PMA group was lower than that in control group (P<0.05). The relative expression of p105/p50 and p65 protein in plasmid group was significantly lower than that in blank group (P<0.05). The expression of p105/p50 and p65 protein in transfection+PMA group [(0.674±0.007), (0.713±0.014)] was significantly higher than that in transfection group [(0.581±0.003), (0.570±0.012)] (P<0.05). Conclusion lncRNA CASC2a may inhibit the proliferation and migration of thyroid papillary carcinoma cells through NF-κB signaling pathway.
ABSTRACT
Objective Forkhead box Q1 (FOXQ1) is highly expressed but its biological role remains unclear in papillary thyroid carcinoma (PTC). This article aims to investigate the effect of FOXQ1-siRAN on the proliferation of TPC-1 cells and its possible mechanism.Methods Synthetic FOXQ1-siRNA and NC-siRNA were transfected into TPC-1 cells by lipofectamineTM2000. The cells were divided into five groups: NC-siRAN, FOXQ1-1, FOXQ1-2, FOXQ1-3 and blank control. After transfection, the expressions of the FOXQ1 mRNA and protein, as well as those of c-Myc and cyclinD1 proteins, were determined by qRT-PCR and Western blot, and the changes in the proliferation of the TPC-1 cells observed by MTT.Results Compared with the blank control and NC-siRAN groups, the FOXQ1-3 group showed a significantly inhibited expression of FOXQ1 mRNA (P<0.05). The protein expression of FOXQ1 was markedly decreased in the FOXQ1-1, FOXQ1-2 and FOXQ1-3 groups in comparison with that in the NC-siRAN group (0.54±0.07, 0.40±0.07 and 0.26±0.06 vs 0.78±0.08, P<0.05). The proliferation of the TPC-1 cells was remarkably lower in the FOXQ1-3 than in the blank control and NC-siRNA groups (P<0.05), and so were the relative protein expressions of c-Myc (0.57±0.04 vs 1.05±0.07 and 0.92±0.06, P<0.05) and cyclinD1 (0.51±0.10 vs 0.94±0.12 and 0.91±0.11,P<0.05).Conclusion Silencing the FOXQ1 gene can effectively inhibit the proliferation of TPC-1 cells in TPC, probably by suppressing the expressions of c-Myc and cyclinD1.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of transplantation of frozen autogenous mandible with delayed implantation.</p><p><b>METHODS</b>Operations were performed to create two defects in the bilateral mandible of 16 dogs. The left defect was grafted by composite transplantation of frozen autogenous mandible (immersed in -196 degrees C liquid nitrogen) with fresh cancellous ilium (composite transplantation group, CTG). The right defect was grafted by fresh ilium (iliac transplantation group, ITG). Three months after transplantation one IMZ TPS dental implant was placed into the graft of each side. At 3, 6, 9, 12 weeks postoperatively, 4 animals were sacrificed respectively and the grafts with dental implant were harvested for gross observation, X-Ray examination and histological evaluation to compare peri-implant bone healing between composite transplantation group and iliac transplantation group.</p><p><b>RESULTS</b>There was no absorbing bone density reducing image of peri-implant at each stage. The quantified X-Ray gray extent displayed obvious variation of interfacial bone density between two kinds of grafts at 3 weeks, 6 weeks and 9 weeks after implantation. The composite transplantation group obviously surpassed the iliac transplantation group. At 12 weeks after the implantation, there was no significant difference between the peri-implant bones of both sides. There was satisfactory osseointegration between the implants and the two kinds of grafts. The healing style of peri-implant bone was similar.</p><p><b>CONCLUSIONS</b>Good osseointegration was performed between the implant and the composite transplantation of frozen autogenous mandible following delayed implantation.</p>