ABSTRACT
OBJECTIVE:To isolate and identify lignans chemical constituents from Coptis chinensis,and to investigate their in-hibitory activities to protein tyrosine phosphatase-1B (PTP1B). METHODS:The acetic ether part of 95% ethanol extract from C. chinensis was isolated by HPLC and gel column chromatography,and the structure of chemical constituents were identified by UV, IR,MS and NMR. Using RK-682 as positive drug,the inhibitory activity to PTP1B was investigated(the concentrations of posi-tive drug and isolated chemical constituents were 0,15,30,50,65,80,100,120,140,170 and 200 μmol/L,respectively). In-hibitory rate and IC50 of them were calculated. RESULTS:5 lignans were isolated,including 9-acetyl lanicepside B,Lanicepside A,Woorenogenin,(+)-isolariciresinol and(+)-lariciresinol gluciside(compound 1-5),and compound 1 and 2 were isolated from C. chinensis for the first time. Compound 1-5 inhibited PTP1B to certain extent in concentration-dependent manner. IC50 of them were 57,49,71,58 and 51 μmol/L,but their effects were not as good as RK-682 (IC50=32 μmol/L). CONCLUSIONS:5 lig-nans have been isolated from C. chinensis and can inhibit PTP1B to certain extent.
ABSTRACT
OBJECTIVE:To observe clinical efficacy and safety of Compound huanglian jiangtang powder for patients with im-paired fasting blood glucose(FBG). METHODS:122 patients with impaired FBG were selected and randomly divided into control group and trial group with 61 cases in each group. Two groups was given healthy education and diet guidance;trial group was giv-en Compound huanglian jiangtang powder 2.5 g orally,qd. Both groups were treated for 4 months. Clinical efficacy of 2 groups was observed and FBG,TC,TG,LDL-C,HbA1c,SOD,MDA and GSH-PX levels and BMI were observed before and after treat-ment. The occurrence of ADR was recorded. RESULTS:Total effective rate of trial group was 77.0%,which was significantly higher than that of control group(24.6%),with statistical significance(P0.05);after treat-ment,above indexes of 2 groups were improved significantly,the trial group was significantly better than the control group,with statistical significance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Compound huanglian jiangtang pow-der is effective improvement of laboratory indexes and safe for impaired FBG.