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Objective To summarize the experience and technique of complex transradial percutaneous coronary intervention ( PCI) using a 4F KIWAMI ST01 catheter with Mother-Child technique for patients with coronary heart disease. Methods Retrospectively analyzed the data from 30 patients who had received PCI using a 4 F catheter with Mother-Child method. Results PCI were performed on a total of 30 patients with complex lesions. Angiographic success was achieved in 100% of lesions. 19 patients were found to have moderate-to-severe calcified lesions, 13 patients had chronic total occlusion ( CTO ) , 11 patients had moderate-to-severe tortuous lesions, and 6 patients had moderate-to-severe angulated lesions. Four patients suffered from in-stent restenosis. 4 F catheter with Mother-Child method was used in all patients, and all 4F catheters successfully passed through the target lesions with all stents implanted in place along the 4 F catheter. There were no 4 F catheter-related complications in all patients. Conclusions Use of 4F catheter with Mother-Child method is safe and effective for patients where stent delivery is difficult by the conventional method.
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Objective To investigate the application and clinical significance of the medial plantar flap on repairing the medial ankle and foot soft tissue defects. Methods A total of 13 patients with the medial ankle and foot soft tissue defects were repaired by the local transferred medial plantar flap alone or in combination with sural neurovascular flap transplantation in the Yuebei People's Hospital Affiliated to the Medical College of the Shantou University from Jun. 2006 to Dec. 2012. Results All patients were followed-up of 4-21 weeks,and the average follow-up periods were 6. 3 weeks. All of the skin and soft tissue defects of the medial ankle and foot were repaired,and there were 2 cases of partial necrosis of flap were repaired after tow times skin grafting operation,and all cases without complications of wound infection or iatrogenic nerve injury. Conclusion The medial plantar flap on treating ipsilateral medial malleolus and foot skin and soft tissue defects is useful and effective with less complications,satisfactory effects,intact the ankle flexor and extensor device,allowing early functional exercise,good functional recovery.
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Objective The purpose of this study is to discuss the expression and pathological signifi-cance of tumor suppressor genes PTEN ,PI3K and AKT in lung cancer .Methods SP immunohistochemical meth-od was used to detect the expressions of PTEN ,PI3K and AKT in 62 cases of lung cancer tissues and 30 cases of cancer adjacent tissues ,and analyzed the correlation and relationship with clinical pathological features .The aim was to explore their roles in lung cancer mechanism .Results The mRNA expression of PTEN in lung cancer tis-sue was significantly lower than that in cancer adjacent tissue ,whereas,the mRNA expressions of PI3K and AKT were significantly higher than that in cancer adjacent tissue (P<0.05).Positive expression rate of PTEN in lung cancer tissues was 48.39%(30/62),which was significantly lower than that of cancer adjacent tissue .The posi-tive expression rate of PI3K and AKT in lung cancer tissue were 79.03%(49/62)and 70.97%(44/62),which was significantly higher than that in cancer adjacent tissue (P<0.01).The expressions of PTEN and PI3K were related with the degree of differentiation ,TNM stage,lymph node metastasis of lung cancer and pathological types . AKT expression was related with the TNM stage ,lymph node metastasis and pathological types of lung cancer ( P<0.05).PI3K and AKT expression were positively correlated (P<0.05),PI3K and PTEN expression were negatively correlated(P<0.05),AKT and PTEN expression were negatively correlated (P<0.05).Conclusion The deceased expression of PTEN tumor suppressor gene in lung cancer and increased expression of PI 3K/AKT,prompt the PTEN and PI3K,and AKT may promote the occurrence ,development and metastasis of lung cancer,and PI3K/AKT signal pathways play an important role in the process ,the gene targeted therapy of lung cancer may provide a new direction .
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BACKGROUND:Ideal models of spinal cord injury should simulate human spinal cord injury, exclude confounding factors affecting effects, and have extensive repeatability. Spinal cord transection model is an ideal choice at present. Nevertheless, the operation is diverse, so therapeutic effects have great differences, and the research results lack of comparability. OBJECTIVE:To compare and analyze behavioral changes and pathological features of rat hindlimb after spinal cord transection by establishing standardized model of transected spinal cord in rats. METHODS:A total of 60 adult female Sprague-Dawley rats were randomly divided into sham surgery group (n=12), conventional spinal cord transection group (n=24) and microscopic spinal cord transection group (n=24). Each group was randomly assigned to 7-day, 14-day and 28-day groups according to time points after model establishment. T9 vertebrae were considered as a center. Sham surgery group underwent laminectomy. Other groups underwent spinal cord transection. Thus, models of acute spinal cord injury were induced. Models in the conventional spinal cord transection group were established by conventional surgical methods. Models in the microscopic spinal cord transection group were established by standardization micromanipulation technique. At 7, 14 and 28 days after model establishment, motor function of hindlimb was evaluated using Basso, Beattie and Bresnahan (BBB) scoring system. Histopathology of transected spinal cord was observed. The thickness of the glial scar, the longitudinal distance between the two stumps, the transverse diameter of the spinal cord cavity and cerebrospinal fluid cyst formation were measured at the transected site of the spinal cord. RESULTS AND CONCLUSION:In the sham surgery group, preoperative and postoperative BBB scores and pathology of the spinal cord did not clearly change. In the conventional spinal cord transection group and microscopic spinal cord transection group, complete paralysis of hindlimb was detected after model establishment. Hindlimb function did not recover in the conventional spinal cord transection group. At 1 to 2 weeks after model induction, spontaneous recovery of hindlimb function was visible in rats of the microscopic spinal cord transection group. Spinal pathology index value was significantly lower in the microscopic spinal cord transection group than in the conventional spinal cord transection group (P<0.01). Pathological observations were not correlated with BBB scores in each group. These data indicated that standardized spinal cord transection method helps to eliminate individual differences and to quantitatively analyze and to compare studies addressing therapeutic effects.
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It is important to reduce blood loss and protect the function of reserved liver during hepatectomy.Selective hepatic vascular occlusion can keep the blood supply of reserved liver and make blood loss less than other methods of blood occlusion, so it helps more to the recovery of liver function.In summary, promotion and application of this technology is worth while.This article reviews the developing progress and the latest studies of selective hepatic blood occlusion to introduce the latest advancement in this area.
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Objective To explore the effect of the surgical technique of jejunum fistula and conventional indwelling nasogastric tube with total gastrectomy. Methods The clinical data of 23 advanced gastric cancer patients with total gastrectomy were retrospetively analyzed. Among 23 cases, 17 received jejunum fistual and 6 received indwelling nasogastric. The time to flatus and the ratio of postoperative complications including throat ache, nausea,pneumonia, anastomotic leak were compared. Results Compared with A group, the incidence of disadvantage spit sputum, dyssomnia, throat ache and nausea in B group reduced significantly, the time to flatus was advanced in B group. And other postoperative complications had no difference between two groups. Conclusion Jejunum fistula was rational, safe and effective in patients with total gastrectomy
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BACKGROUND: Physical training induces adaptation changes on physiological function and anatomic structure of body, which significantly exist in individuals. Individual variation responses to physical training is determined by gene polymorphism. OBJECTIVE: To summarize the research progress on the association between gene polymorphism and exercise training sensitivity in recent years. RETRIEVAL STRATEGY: A computer-based search of Pubmed database was undertaken to identify the articles published from January 2002 to December 2006 with the key words of "gene polymorphism, endurance training, strength training, aerobic training" in English. Simultaneously, China Journal Full-text Database was searched for the relevant articles published from January 2002 to December 2006 with the same key words in Chinese. Totally 58 relevant articles were collected after the first trial. Inclusive criteria: ①articles on association between gene polymorphism and endurance training, ②articles on association between gene polymorphism and strength training, ③articles on association between gene polymorphism and hypoxia training. LITERATURE EVALUATION: The articles collected were prospective cohort studies on association of gene polymorphism with some physiological and biochemical parameters response to physical training. DATA SYNTHESIS: ①The individual variation responded to physical training in physiological and biochemical parameters are mostly determined by genetic factors, such as gene polymorphism.②Recently, there are lots of studies on association of gene polymorphism with physiological and biochemical parameters response to endurance training, strength training and hypoxia training.③Mostly studies indicate that there are associations among physiological and biochemical parameters response to endurance training, strength training and hypoxia training and I/D polymorphism in ACE gene, C1772T in HIF-1? gene and so on. CONCLUSION: Although gene polymorphism is a genetic marker of determining the physiological phenotype of human, the various genetype of the same gene polymorphism can cause different sensitivity change of physiological phenotype in the different races response to training. With the research progress on association between gene polymorphism and physical training sensitivity, it will indicate its related mechanism.