ABSTRACT
Objective To observe morphological changes of hippocampal CA1 neurons induced by ischemic acute kidney injury (IAKI) in rats.Methods After successful preparation of rat model of IAKI, the morphological changes of hip-pocampal CA1 neurons were observed by light microscope and electron microscope. The expressions of polyADP-ribose polymerases (PARP)-1 and caspase-3 in hippocampal CA1 neurons were detected by immunohistochemical (IHC) staining and Western blot assay.Results Pyknotic neurons in hippocampal CA1 area were found after 60 min ischemia and 24 h re-perfusion in kidney. Results of electron microscope showed that swollen mitochondria and dilated endoplasmic reticule in cy-toplasm and shrinkage nucleus with no pyknotic chromatin in the pyknotic neurons. IHC staining showed the negative cas-pase-3 staining and positive PARP-1 staining in pyknotic neurons. Conclusion The pyknotic neurons induced by IAKI might be mediated by PARP-1.
ABSTRACT
PURPOSE: Synuclein-gamma (SNCG), which was initially identified as breast cancer specific gene 1, is highly expressed in advanced breast cancers, but not in normal or benign breast tissue. This study aimed to evaluate the effects of SNCG siRNA-treatment on breast cancer cells and elucidate the associated mechanisms. METHODS: Vectors containing SNCG and negative control (NC) siRNAs were transfected into MDA-MB-231 cells; mRNA levels were determined by real-time polymerase chain reaction. Cell proliferation was evaluated using the MTT assay, cell migration was assessed by the Transwell assay, apoptosis and cell cycle analyses were conducted with the flow cytometer, and Western blot analysis was performed to determine the relative levels of AKT, ERK, p-AKT, and p-ERK expression. RESULTS: SNCG mRNA levels were significantly reduced in MDA-MB-231 cells transfected with SNCG siRNA. Our results indicate that in SNCG siRNA-treated cells, cell migration and proliferation decreased significantly, apoptosis was induced, and the cell cycle was arrested. Western blot analysis indicated that the protein levels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups. CONCLUSION: SNCG siRNA could decrease the migration and proliferation of breast cancer cells by downregulating the phosphorylation of AKT and ERK.