ABSTRACT
Objective To analyze the distribution of various genotypes of human cytomegalovirus glycoprotein N ( HCMV gN) in patients with HIV infection; to investigate the effects of HCMV-HIV co-in-fection on disease progression and the relationships between HCMV gN genotypes and disease progression. Methods Patients with active HCMV infection were screened out from 359 patients with HIV infection by using the pp65 antigenemia assay.The genes encoding HCMV gN ( UL73 ) were amplified by nested PCR ( nPCR) .The amplicons were digested by restriction enzymes including MboⅠ, ScaⅠ and SalⅠ.Then, the restricted fragment length polymorphisms were further analyzed on 4%agarose gel.The relationships be-tween HCMV genotypes and the morbidity and mortality of acquired immune deficiency syndrome ( AIDS ) were investigated via a prospective study.Results Among the 359 patients with HIV infection, 28 subjects were positive for the HCMV pp65 antigenemia assay.The HCMV gN genotypes in 20 patients with active HCMV infection were distributed as: gN-3a (4/20, 20%), gN-1 (4/20, 20%), gN-4d (1/20, 5%), gN-4b (1/20, 5%) and mixed infection (10/20, 50%).Patients with HCMV-HIV co-infection were more likely to develop AIDS during the follow-up period (RR=9.78).Patients harboring HCMV gN-1 and gN-4 genotypes would seem likely to have 4.6 times of chance leading to AIDS-associated death than those harbo-ring other HCMV gN genotypes.Conclusion HCMV infection ( especially gN-1 and gN-4 genotypes) might accelerate the progression of HIV infection.
ABSTRACT
This study was aimed to develop a simultaneous determination of zinc and cadmium with carbon fiber electrode by linear sweep stripping voltammetry. Effects of analytical base solution, accumulation potential, ac-cumulation time and scanning velocity were also investigated on the determination. The results showed that in 1 mol·L-1 H2SO4 medium solution, zinc and cadmium had good electrochemical response on the carbon fiber electrode and appeared sensitive anodic stripping peaks at-0.97 V and-0.77 V, respectively. The anodic stripping peak cur-rents and concentrations of zinc and cadmium showed good linear relationships, with correlation coefficients R2 of 0.996 5 and 0.995 4, respectively. The detection limits of zinc and cadmium (S/N=3) were 1.5×10-9 mol·L-1 and 1.0×10-10 mol·L-1, respectively. The average recoveries of zinc and cadmium were 98.9%and 98.1%with the rela-tive standard deviations (RSD) of 2.72%and 2.45%(n=6), respectively. It was concluded that the method of simul-taneous determination of zinc and cadmium with carbon fiber electrode was simple, sensitive and accurate.
ABSTRACT
To solve the problems in the materials,spirit and abiligy of the students from the article poor family,claims to construct a fire-new university students subsidizing system from the trinity of ‘subsidize,encourage,quality development’ by the three sides:the multi-funding channels,multi-dimensional motivational strategies and diverse quality development training,aiming at enhancing the effect of the funded job in high school.