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ObjectiveTo investigate the clinical characteristics of leprosy-related neuritis with bullous pemphigoid after treatment of paucibacillary leprosy. MethodsThe treatment of leprosy reaction combined with bullous pemphigoid of a cured case of leprosy was analysed. ResultsFive years after standard treatment for leprosy， erythema and vesicles appeared in the limbs without obvious inducement， and the disease became more and more severe. With clinical diagnosis and pathological examination， pemphigoid was confirmed， and the patients were given hormone treatment for leprosy reaction and anti-immunotherapy， as well as symptomatic supportive treatment. ConclusionLeprosy reaction and pemphigoid are both related to immunity， but the occurrence of both at the same time is relatively rare， so in the clinical process we should attach great importance to early detection， early diagnosis and prompt treatment to prevent further harm to the patient.
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The work explored the DEHP migration parameters in PVC infusion in clinic,based on the previous research on the test model of DEHP migrated from PVC infusion,to assess the safety of PVC infusion.The leaching solution samples in different conditions were evaluated by analysis of the DEHP in leaching solution using GC-MS under simulated clinical transfusion way.The release behavior of DEHP was significantly affected by the storage time,storage temperature,surrounding temperature,dripping speed,sterilization process,volume of the leaching solution,and the property of the leaching solution.
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Diethylhexyl Phthalate , Pharmacokinetics , Gas Chromatography-Mass Spectrometry , Plasticizers , Pharmacokinetics , Polyvinyl Chloride , Pharmacokinetics , TemperatureABSTRACT
As we all know, DEHP is seriously harmful to human health and consequently has been acquired critical attention. DEHP is able to migrate from PVC medical devices for the non-chemically bound to PVC, thus contact with user and patient. The DEHP migration is influenced by various parameters. In order to assess the security of PVC-tubes medical devices scientifically of DEHP migration, we develop an experimental model by analyzing the parameters comprehensively and systematically, taking into account the clinical practices. For example, assessing the security of DEHP migration from infusion sets by utilizing this model.
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Humans , Diethylhexyl Phthalate , Equipment and Supplies , Models, Theoretical , Plasticizers , Polyvinyl ChlorideABSTRACT
Objective To explore the application and effect of international patient safety goals in nursing management at health management center under the standard of American Joint Commission on Accreditation of Healthcare Organizations. Methods From January 2012 to January 2013, six patients' safety goals of the Joint Commission International (JCI) standard were executed to the nursing management at the health management center. Compare the relevant nursing safety monitoring indicators and customer satisfaction one year before and after the execution of JCI standard. Results Our department was certified by JCI in February 2013. After 1 year of implementation of JCI standard, the coincidence rate of identification of customers for health examination increased from 85.62% to 98.92% (χ2 = 297.264, P <0.05) and the accuracy rate of management of critical values increased from 89.00% to 96.00% (χ2 =84.757, P<0.05). The accuracy rate of disposal of significant abnormalities of check-up increased from 90.67%to 96.50%(χ2=69.184, P<0.05). The coincidence rate of high-risk drugs storage increased from 90.17%to 96.50%(χ2=77.357, P<0.05). The hand hygiene compliance rate increased from 75.25%to 88.63%(χ2=145.047, P<0.05). The awareness rate of adverse events report was raised from 84.00%to 96.67% (χ2 = 220.486, P < 0.05). The satisfaction rate of customers at the health management center increased from an average of 93.94 points to 98.66 points (t=-7.903, P<0.05). Above comparisons are all statistically significant. Conclusions The effective implementation of JCI standard patient safety goals helps to achieve scientific, standardized, orderly and detailed nursing management, optimize nursing work processes, standardize operations, reduce nursing risk and improve nursing quality, which makes a contribution to improve patients′satisfaction and gain patients′trust.
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Objective To explore the application and effect of international patient safety goals in nursing management at health management center under the standard of American Joint Commission on Accreditation of Healthcare Organizations. Methods From January 2012 to January 2013, six patients' safety goals of the Joint Commission International (JCI) standard were executed to the nursing management at the health management center. Compare the relevant nursing safety monitoring indicators and customer satisfaction one year before and after the execution of JCI standard. Results Our department was certified by JCI in February 2013. After 1 year of implementation of JCI standard, the coincidence rate of identification of customers for health examination increased from 85.62% to 98.92% (χ2 = 297.264, P <0.05) and the accuracy rate of management of critical values increased from 89.00% to 96.00% (χ2 =84.757, P<0.05). The accuracy rate of disposal of significant abnormalities of check-up increased from 90.67%to 96.50%(χ2=69.184, P<0.05). The coincidence rate of high-risk drugs storage increased from 90.17%to 96.50%(χ2=77.357, P<0.05). The hand hygiene compliance rate increased from 75.25%to 88.63%(χ2=145.047, P<0.05). The awareness rate of adverse events report was raised from 84.00%to 96.67% (χ2 = 220.486, P < 0.05). The satisfaction rate of customers at the health management center increased from an average of 93.94 points to 98.66 points (t=-7.903, P<0.05). Above comparisons are all statistically significant. Conclusions The effective implementation of JCI standard patient safety goals helps to achieve scientific, standardized, orderly and detailed nursing management, optimize nursing work processes, standardize operations, reduce nursing risk and improve nursing quality, which makes a contribution to improve patients′satisfaction and gain patients′trust.
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Objective To introduce transfusion monitoring system to optimize nursing flow under field conditions.Methods The organization and arrangement of nursing staffs were optimized with high-precision droplet sensor and transfusion monitoring system.A post of caring nurse was added to execute mental healthcare of the patient.Results Humanistic care was carried out by the caring nurse with daily nursing completed,and there's no need for additional nursing staff.Conclusion The transfusion monitoring system is rapid in deployment and withdrawal,optimizes nursing flow and enhances nursing quality,and thus is worthy promoting widely.
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Objective To assess the effect of a noninvasive equipment (the human biological stimulation feedback instrument) for the screening of metabolic syndrome (MS). Methods The samples were selected from the International Health Care Center of the Second Affiliated Hospital of Zhejiang University College of Medicine. From January to December 2012, according to the inclusion criteria, a total of 5674 consecutive participants (3 437 men, 2 237 women) without medical history of diabetes mellitus underwent general health screening in this hospital, which included questionnaire investigation, all MS components and the risk assessment of the human biological stimulation feedback instrument. MS was defined according to 2004 the Chinese Diabetes Society (CDS) criteria. Results The 5 674 subjects were divided into three groups in accordance with the human biological stimulation feedback instrument risk score: the normal group, metabolic abnormalities low risk group, metabolic abnormalities high risk group. The detection rate of MS corresponding to the three groups was 8.73% (251/2 876), 29.32% (612/2 087), 32.77% (233/711), respectively (trend χ2=372.14,P<0.001). With the increase of risk score, the detection rate of MS increased. A total of 1 096 (male 879, female 217) were diagnosed MS by CDS criteria. The area under the curve (AUC) of the test was 0.674 (95%CI 0.658-0.691, P<0.000 1)for predicting MS. The corresponding cutoff value was 25.00%, the sensitivity, specificity, positive predictive value and negative predictive value of diagnosis of MS was 77.80%, 56.00%, 30.20%and 91.30%, respectively;409 subjects who had no history of MS and diabetes mellitus follow up for two years, the AUC of the test was 0.719 (95%CI 0.644-0.794)for predicting MS. The corresponding cutoff value was 25.00%, too. And the sensitivity and specificity of diagnosis of MS was 78.00%and 59.80%, respectively. The detection rate of MS was positively correlated with the risk score. Conclusion We found that the human biological stimulation feedback instrument is a potential screening approach for early predicting MS in health checkup with simple and noninvasive procedure and high negative predictive value, although its diagnostic efficiency is not so good. And it might have a warning effect on the progress of MS.
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Inorganic layered materials with the open structure of low dimension and good biological compatibility and stability are considered as one of the most promising materials for immobilizing biomolecules, which can not only adjust the spacing of layers according to guest molecular size to prevent leakage of guest molecule, but also reduce the effects of surrounding elements on immobilized molecules significantly. Moreover, they can promote electron transfer between enzyme and the electrode. Therefore, the inorganic layered materials have great application potential in electrochemical sensors. This paper reviews the ways of immobilizing guest molecules of inorganic layered materials and the applications and development prospects of the different types of inorganic layered materials including cationic, anionic and nonionic inorganic layered materials in electrochemical sensor.
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Based on the competition reaction of target protein, aptamer probe, padlock probe and complementary sequence, a highly sensitive fluorescent aptasensor was developed in this study in combination with rolling circle amplification. In the absence of target protein, the ligation-rolling circle amplification reaction was repressed because the complementary sequence hybridized with aptamer probe to form double-stranded duplex. While in the presence of target protein, the target molecules bound specifically with aptamer probe, inducing displacement of the complementary sequence and hybridization with padlock probe. The padlock probe was circularized with the assistance of E. coli DNA ligase, and the rolling circle amplification process could be accomplished by Phi 29 DNA polymerase. The amplification product contained thousands of repeated sequences which could hybridize with the loop of molecular beacon ( the detection probes) , resulting in a significant fluorescence signal. The effects of length of complementary DNA ( CDNA ) sequence and concentration of padlock probe were investigated. Under the optimized experimental conditions, the model target protein thrombin could be highly sensitively detected by the proposed aptasensing system in a linear range of 0 . 067-32 . 4 nmol/L with a detection limit of 0 . 03 nmol/L ( approximately 90 amol target molecules). Moreover, the presented sensing method was universal for other target analysis by skillfully design of the sequence of aptamer probe and related oligonucleotides.
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Objective To explore the correlation between gratitude and turnover intention of nursing staff.Methods A total of 660 clinical nurses from three general hospitals in Hangzhou city were subject to questionnaire survey for the correlation.Results The multidimensional gratitude totaled 128.33±32.67,at a medium or low level; their turnover intention totaled 14.59 ± 2.26,at a medium level.The multiple factor variance analysis showed statistically significant negative correlations between the three dimensions of gratitude(a sense of rich,simple thanks,and thanks to others)and turnover intention.Conclusion Related departments are recommended to pay close attention to nurses' gratitude and carry out gratitude education,so as to decrease their turnover intention,and keep the career stability of nurses.
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A novel and highly sensitive voltammetric enzyme-linked immunosensor was developed based on tyramine) oxidation deposition. It was shown that gold nano-particles(colloid Au) could be used as a platform to immobilize antibodies by adsorption. By a sandwich immunossary format with goat-anti-human IgG labled Horseradish peroxidase(HRP) as the second antibody and catalytic amplification by biotin-tyramine, the immunosensor′s) catalytic ability to hydrogen peroxide increased nearly 20 times), the sensor exhibitd a linear response to human IgG in the concentration range from 1.5 μg/L-22 mg/L, and the detection limit was 0.1 μg/L), the regression equation could be expressed as Δi_p(μA) =2.8859c(mg/L)+17.152 with a correlation) coefficient of 0.9872. The immunosensor can be used to quantitatively determine hIgG in the sample) of human serum).
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A novel simple,sensitive fluorescence immunosensing method based on aptamer-plasmid complex amplification was developed. This method utilized the specific recognition between antibody and antigen as well as aptamer-plasmid complex and the intercalation of fluorescence dye SYBR Green Ⅰ in the groove of duplex plasmid DNA in detection of Platelet-Derived Growth Factor BB (PDGF-BB). The immunoassay was performed in the microtiter wells in which rabbit anti PDGF-BB antibody was immobilized. The PDGF BB analyte was captured by the primary antibody and then sandwiched by the aptamer-plasmid DNA complex. The introduction of fluorescence dye SYBR Green Ⅰ allows for the detection of the sandwiched immunocomplex of antibody/anigen/aptamer-plasmid complex. Under the optimized conditions of salt concentration,ratio of aptamer to PUC19,and SYBR Green Ⅰ concentration,the proposed method offers a linear detection range from 0.2 μg/L to 200 μg/L with a detection limit of 0.1μg/L.
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In this article， several kinds of common immunosensors and the development of their transducers are introduced． Meanwhile， some problems in the fabrication of immunosensor such as immobilization method and reproduction are discussed．