ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Qingkailing injection (QKLI) on complement and RBL-2 H3 cells in virto.</p><p><b>METHOD</b>The mixture of human serums and QKLI were incubated for 30 min in vitro and then the content of SC5 b-9 in the mixture was determined by ELISA. RBL-2 H3 cells were cultured and treated by QKLI. Beta-heosaminidase release rate was measured by coloration method. The content of histamine in supernatant was tested by ELISA.</p><p><b>RESULT</b>The QKLI can reduce the content of SC5 b-9 (P<0.05) and promote the release of beta-heosaminidase and histamine significantly (P<0.05).</p><p><b>CONCLUSION</b>QKLI didn't induce the complement activation, but induced the release of beta-heosaminidase and histamine directly. Therefore, the clinical adverse reactions of QKLI in clinic may be pseudoallergy which had no relation with the activation of complement system.</p>
Subject(s)
Adolescent , Adult , Animals , Humans , Rats , Young Adult , Cell Degranulation , Cell Line, Tumor , Chemistry, Pharmaceutical , Complement System Proteins , Metabolism , Drugs, Chinese Herbal , Chemistry , Pharmacology , Hexosaminidases , Bodily Secretions , Histamine Release , InjectionsABSTRACT
<p><b>OBJECTIVE</b>To optimize the ultrahigh pressure extraction (UPE) process of polydatin and resveratrol from Polygonum cuspidatum by using uniform design.</p><p><b>METHOD</b>On the basis of single factor screening, the uniform design was adopted for getting the optimal technique parameters. The optimum result of UPE was compared with conventional extractions.</p><p><b>RESULT</b>The optimal conditions of UPE for polydatin and resveratrol were that the solvent was 55% ethanol, the ratio of solvent to material( mL: g) was 30, the extraction pressure was 170 MPa, and the extraction time was 120 second. With this extracting process, the extraction yield of polydatin and resveratrol were 14.29 and 2.53 mg x g(-1), respectively. The extraction yield of polydatin was 46.1% higher than the heat reflux extraction, 6.4% higher than the ultrasonic extraction and 28.5% higher than the microwave extraction, while the yield of resveratrol was 67.5% higher than the heat reflux extraction, 29.7% higher than the ultrasonic extraction and 24.6% higher than the microwave extraction, respectively.</p><p><b>CONCLUSION</b>As a novel extraction technology for Chinese herbal medicine, the UPE procedure has higher extraction yield, lower extracting temperature, shorter extacting time and less power consumption. The UPE has provided a brand-new method for extraction of polydatin and resveratrol from P. cuspidatum.</p>