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Objective:To investigate the expression of miRNA-4766-5p (miR-4766-5p) in ovarian cancer tissues, the effect of miR-4766-5p on the proliferation and invasion of ovarian cancer cells in vitro and its related mechanism.Methods:The cancerous tissues and adjacent tissues of 32 patients with ovarian cancer who underwent surgical treatment in the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology from February 2019 to December 2020 were selected, as well as the ovarian cancer cell lines (A2780, OC3, SKOV-3. HO-8910, and OVCAR-3) and normal ovarian epithelial cell line IOSE80 were selected for subsequent experiments. Real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) was used to detect the relative expression of miR-4766-5p in each cell line and ovarian cancer tissues. The cell line with the lowest relative expression of miR-4766-5p was taken as the experimental subject, and the negative control plasmid (control group) and the plasmid expressing miR-4766-5p (miR-4766-5p group) were transfected respectively into the ovarian cancer cells. CCK-8 method and Transwell experiment were used to detect the effect of overexpression of miR-4766-5p on the proliferation and invasion ability of the selected cells. PITA and starBase V2.0 softwares were used to predict the target genes of miR-4766-5p, and the dual luciferase reporter gene method was used for verification. qRT-PCR and Western blot were used to detect the effect of overexpression of miR-4766-5p on target gene expression of the selected cell lines.Results:The relative expressions of miR-4766-5p in ovarian cancer tissues and adjacent tissues were 1.06±0.17 and 5.25±0.70, respectively, and the difference was statistically significant ( t = 5.86, P < 0.01). Compared with IOSE80 cells, the relative expression of miR-4766-5p in all ovarian cancer cell lines decreased (all P < 0.01), and the relative expression of miR-4766-5p in OC3 cells was the lowest, so this cell line was used for subsequent experiments. The result of CCK-8 method showed that the absorbances of OC3 cells in the miR-4766-5p group were lower than those in the control group after 1 d, 2 d and 3 d of cell culture (all P < 0.05). The result of Transwell experiment showed that the number of penetrating cells in OC3 cells of the miR-4766-5p group and the control group were 25±6 and 86±11, respectively, and the difference was statistically significant ( t = 4.77, P < 0.01). PITA and starBase V2.0 softwares predicted that miR-4766-5p may have binding sites with microtubule unstable protein 1 (STMN1) mRNA; the result of dual luciferase reporter gene showed that the target gene of miR-4766-5p may be STMN1. The relative expression of STMN1 mRNA in OC3 cells of the miR-4766-5p group and the control group were 0.28±0.05 and 1.00±0.05, respectively, and the difference was statistically significant ( t = 10.47, P < 0.01). Compared the control group, the expression of STMN1 protein in the miR-4766-5p group decreased, the expression of epithelial cell phenotype protein β-catenin increased, the expression of mesenchymal cell phenotype protein Snail decreased, and the expressions of cyclin CDK2 and cyclin E decreased. Conclusion:miR-4766-5p is under-expressed in ovarian cancer tissues and ovarian cancer cell lines, and miR-4766-5p may inhibit the proliferation and invasion of ovarian cancer cells by down-regulating the expression of its target gene STMN1.
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Objective To investigate the effect of quinone oxidoreductase 1 (NQO1) and quinone oxidoreductase 2 (NQO2) expression in the prognosis of ovarian carcinoma.Methods The two online databases were used to perform the online analysis on the NQO1 and NQO2 mRNA levels and prognosis of ovarian cancer patients,1 306 cases of ovarian cancer were performed the Kaplan-Meier analysis by using the KaplanMeier plotter (K-M plotter);578 cases of ovarian cancer in the TCGA database were performed the univariate COX regression survival analysis.Results The K-M plotter analysis showed that the NQO1 expression level had no obvious correlation with the prognosis of ovarian cancer (P>0.05),the higher the NQO2 level,the better the prognosis (HR=0.83,P=0.006 2).The COX regression survival analysis showed that the NQO1 expression level had no obvious correlation with the prognosis of ovarian cancer(P>0.05),the higher the NQO2 level,the better the prognosis (P=0.038 29).Conclusion NQO2 expression level has no obvious correlation with the prognosis of ovarian cancer,moreover the higher the NQO2 expression level,the better the clinical prognosis of ovarian cancer.
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OBJECTIVE@#To compare the CT scan results in patients with deviation of nasal septum and normal adults and to explore its values in evaluating the state of nasal ventilation.@*METHOD@#Twenty-six patients with deviation of nasal septum and 15 normal people were recruited in the study. The nasal airway from anterior nostril to posterior nostril was evaluated with PhlipsTomos scan AV Expander Elspiral scanner. The indexes of nasal ventilation was measured with the image-analysis software. The results of CT scan between patients with deviation of nasal septum and normal people was compared.@*RESULT@#(1) There was obvious difference between patients with deviation of nasal septum and normal people in the NMCSA, IMCSA, DNCAT and DICAT. There was obvious difference between patients with deviation of nasal septum with obstruction in the opposite side and normal people in the ONMCSA and ODNCAT. There was obvious difference between patients with deviation of nasal septum without obstruction in the opposite side and normal people in the ODNCAT. (2) In the patients with deviation of nasal septum, between nasal minimal cross sectional area and inferior turbinate's maximum cross sectional area, there is obvious relation, r = -0.394, P < 0.05.@*CONCLUSION@#CT is one kind of objective inspection method in evaluating the state of nasal ventilation. With the measurement of the nasal morphology and functional index, we can understand the state of nasal ventilation more comprehensively. And with this, the treatment and diagnosis of disease with nasal obstruction will be more scientific.
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Case-Control Studies , Nasal Obstruction , Diagnostic Imaging , Nasal Septum , Congenital Abnormalities , Diagnostic Imaging , Pulmonary Ventilation , Tomography, X-Ray ComputedABSTRACT
Objective To introduce a modified intraoral fixed appliance for maxillary protraction and to investigate the dentofacial changes of anterior crossbite after the therapy. Methods Twelve patients with anterior crossbite were treated with modified protraction therapy. The cephalometric analysis was used to evaluate the changes. Results After active treatment, the overjet correction was 6.55 mm. A point moved forward 2.6 mm. Pg dislocated backward 5.65mm with MP-FH increased 1.7?. Esthetics was greatly enhanced. Conclusion The modified maxillary protraction is recommended for easy fabrication, good retention, wearing comfortably and cleaning easily. Esthetics is greatly enhanced.