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AIM: Given the biofilm formation abili- ty of different ST-type Klebsiella pneumoniae, our study was aimed at exploring the inhibitory and clearance of azithromycin combined with levofloxacin on the biofilm of Klebsiella pneumoniae of different ST-types and providing a new strategy for the prevention of biofilm formation in the treatment of post-infectious Klebsiella pneumoniae. METHODS: 9 strains of Klebsiella pneumoniae from all susceptibility groups, 19 strains of Klebsiella pneumoniae producing extended-spectrum β - lactamases (ESBLs), and 37 strains of Carbapenem-resistant Klebsiella pneumoniae (CRKP) were randomly collected from the samples of patients hospitalized in the First Affiliated Hospital of Wannan Medical College from August 2019 to November 2021. The isolates were identified using VITEK MS IVD KB V3.2 and VITEK 2-Compact 60. Multilocus sequence typing (MLST) was performed to analyze the homology of each strain; crystal violet staining was used for semi -quantitative detection of biofilm to compare the differences in biofilm formation ability between different ST-type Klebsiella pneumoniae. Different ST-type strains were selected, and the partial inhibitory concentration index (FICI) was calculated by micro broth dilution method to judge the combination effect and select the optimal combination concentration; crystalline violet staining method was used to investigate the inhibition and clearance effect of azithromycin combined with levofloxacin on the biofilm of different ST-type Klebsiella pneumoniae; laser scanning confocal fluorescence microscopy was used to observe the structural changes of the biofilm of Klebsiella pneumoniae before and after the effect of the antibacterial drugs. RESULTS: MLST typing results showed that the sensitive group of Klebsiella pneumoniae strains had 8 sequences such as ST86, ST727, etc., the ESBLs group strains belonged to 14 sequence types such as ST15, ST37, ST11, etc., of which ST15 accounted for 26.32% (5 / 19). The CRKP group strains belonged to 9 sequence types such as ST11, ST15, ST656, etc., of which ST11 accounted for 48.65% (18/37), ST15 accounted for 27.03% (10/37); ST15 (ESBLs), ST11 (CRKP), ST15 (CRKP) type Klebsiella pneumoniae biofilms all reached maturity on the 5th day, the ST15 (ESBLs) group had a stronger ability to produce material to be membranous than the ST15 (CRKP) group. The ST11 (CRKP) group had a stronger ability to produce material to be membranous than the ST15 (CRKP) group (Pazithromycin>levofloxacin, and the highest clearance rate was 44.79%. CONCLUSION: There are differences in biofilm formation ability between different ST-type Klebsiella pneumoniae, and azithromycin combined with levofloxacin has a better inhibitory effect on different ST-type Klebsiella pneumoniae biofilm, conbined application can be used in the treatment of biofilm infections early stage.
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AIM: To study the inhibitory and scavenging effects of ambroxol combined with levofloxacin on the bacterial biofilm of Klebsiella pneumoniae, and to provide a new strategy to treat and antagonize the formation of the biofilm. METHODS: We collected Klebsiella pneumoniae of different resistance and divided them into sensitive group (wild bacteria group), ESBLs group and CRKP group with 15 strains in each group and performed biological semi-quantitative detection of its biofilm by crystalline violet staining method. After selecting 3 strains with similar membrane yields from each group, we determined the minimum inhibitory concentration (MIC) of ambroxol and levofloxacin against Klebsiella pneumoniae by micro broth dilution method.In addition, we determined the effects of ambroxol in different concentrations on the MIC of levfloxacin by the micrdilution checkerboard techniques and calculated the partial inhibitory concentration index (FIC) to determine the joint effect and select the best synergistic concentration. Finally, the effects of ambroxol and levofloxacin in different concentrations on the inhibition formation test and removal test of Klebsiella pneumoniae biofilm were observed by crystal violet method combined with confocal laser scanning microscopy. RESULTS: We found that all three groups of bacterial biofilms became mature on the 5th day, and the sensitive group was easier to form and produce biofilm more than the ESBLs and CRKP groups (F=3.725, P=0.032). It was showed that the geometric average of levofloxacin MIC value in the three groups decreased significantly. And the selected strains all showed a synergistic effect on the two-drug combination.In the biofilm test, as the concentration of ambroxol increased, its inhibition rate reached more than 75%, but its biofilm removal rate did not reach 70%. CONCLUSION: Our study support that ambroxol combined with levofloxacin can inhibit the bacterial biofilm of Klebsiella pneumoniae early, and its optimal synergistic concentration is 0.49 mg/mL (ambroxol) + 4 μg/mL (levofloxacin).
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Objective To observe the effect and mechanism of periosteal pressure kneading plus resistance exercise in improving obstructive sleep apnea/hypopnea syndrome (OSAHS).Methods Thirty-two patients with moderate OSAHS were divided randomly into a kneading plus resistance training group and a conventional drug group,each group with 16 patients.The training group was given periosteal pressure kneading plus resistance exercise while the drug group received only conventional oral treatment with drugs.Before and after treatment,polysomnography (PSG),Thera-Band elastic bands and a PWS angle measuring instrument were used to observe PSG parameters around the neck plus the muscle strength and cervical activity of both groups.Results In the training group the following components of the PSG index had improved significantly compared with before treatment:respiratory events (AHI),sleep structure (SS),awakening number (AN),non-rapid eye movement (NREM),rapid eye movement (REM),mean oxyhemoglobin saturation (MSaO2) events,lowest oxyhemoglobin saturation (LSaO2),and the index of oxyhemoglobin saturation decrease (ODI).In the drug group only the LSaO2 and ODI had improved significantly compared with before treatment.In terms of the PSG parameters,the training group demonstrated significantly greater improvement than the drug group.After treatment,the training group's average residence time in neck load pulling was extended significantly while that of the drug group showed no significant change.The difference between the groups was statistically significant.After the treatment the training group's average activity of the cervical spine was also significantly increased,and again the drug group showed no significant improvement.Conclusion Skeletal lesions in peripheral airways may be an important factor in the formation of OSAHS.Periosteum kneading with resistance exercise has an obvious curative effect on OSAHS.
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Neonatal jaundice is a common neonatal disease. Severe jaundices lead to kernicterus that affects intellectual development of infants or even causes death. Timely and early prediction is vital to the treatment and prevention. This paper presents a jaundice predictor, which uses C8051F020 as the core of single-chip microcomputer (SCM) system with prediction algorithms proven by a large number of clinical trials. The jaundice predictor can reduce the incidence rate of jaundice, alleviate the condition of infants with jaundice, improve the quality of perinatal, with predicting pathologic neonatal jaundice effectively and calling attention to the prophylactic treatment. In addition, compared with the existing transcutaneous jaundice meters, the new predictor has a smaller size, a lighter weight, more user-friendly, and easier to use by hand-holding.
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Humans , Infant, Newborn , Algorithms , Bilirubin , Blood , Equipment Design , Methods , Jaundice, Neonatal , Blood , Diagnosis , Microcomputers , Photometry , MethodsABSTRACT
<p><b>OBJECTIVE</b>To investigate the genetic diversity and structure of Dendrobium huoshanense, a (CT)n enriched microsatellite library was constructed using a magnetic beads enrichment procedure.</p><p><b>METHOD</b>The 3'-biotinylated oligonucleotide probe was used to hybridize with the digested D. huoshanense genomic DNA fragments whose both ends were ligated with adaptors. The hybridized complex was then combined with the streptavidin-coated magnetic beads. The captured microsatellite fragments were eluted, collected and cloned into pMD19-T vector. The recombinant plasmids were transformed into Escherichia coli DH5alpha competent cells. The clones that yielded two or more bands contained microsatellite fractions. Positive clones were screened and sequenced. Thirty pairs of primers were designed and synthesized. Polymorphism at each locus was determined using 24 individuals from a natural population from Huoshan county town in Anhui province.</p><p><b>RESULT</b>Twelve polymorphic microsatellite loci from the microsatellite-enriched genomic library were newly developed across 24 D. huoshanense individuals. In total, 65 alleles were identified, and the number of alleles per locus ranged from 2 to 8. The mean observed and expected heterozygosities were 0.500 and 0.638, respectively. Two loci significantly deviated from Hardy-Weinberg equilibrium (P<0.05), which could be due to the presence of null alleles. Furthermore, three of twelve loci showed significant linkage disequilibrium (P<0.05).</p><p><b>CONCLUSION</b>These results suggest that the identified polymorphic microsatellite markers will be useful in population genetic studies of D. huoshanense.</p>
Subject(s)
Dendrobium , Genetics , Genomic Library , Microsatellite Repeats , Molecular Sequence Data , Nucleic Acid Hybridization , Polymorphism, GeneticABSTRACT
Objective To observe clinical effects of round ligament ventrosuspension or shortening round ligament operation by laparoscopy on treating pelvic congestion syndrome (PCS) after tubal ligation. Methods Thirty patients with PCS were treated in round ligament ventrosuspension or shortening round ligament operation by laparescopy. Follow-up was started from the third month after operative procedure to find out patients' symptoms ameliorated situations. Results Symptoms of all patients were ameliorated in various degrees after operations. The cure rate of the cases was 73.3%(22/30), and the rate of improvement was 20.0% (6/30), and the rate of inefficiency was 6.7% (2/30), and total effectiveness rate was 93.3% (28/30). Conclusions Laparoscopy is chosen to be a routine and first diagnosis or treatment means. Round ligament ventrosuspension or shortening round ligament operation by laparoscopy is a simple, high effective way of treating PCS after tubal ligation.
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[Objective]To study better treatment of anal itching.[Method]Randomly divide 64 cases into 2 groups;the control group 32 cases take peri-anal sealing,the treatment group 32 combined with Chinese drugs fume-cleansing and seated bath,half month as a course,make statistics after 2 courses,take itching disappearing and injured skin recovered normal as evaluation standard.[Result]In treatment group,22 cases were cured,5 had marked effect,3 were better,2 had no effect,the total effective rate was 94%;for control one,they were respectively 10,3,7,12 and 52%;both groups comparison was of statistical meaning(P