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@#Lung cancer is the malignant tumor with the highest incidence rate in men and the highest mortality rate in men and women in China, and the incidence and mortality rates are still increasing. Lung cancer screening is an important initiative for early detection of lung cancer and improvement of prognosis. The National Comprehensive Cancer Network (NCCN) updates the NCCN Clinical Practice Guidelines for Lung Cancer Screening annually, and the 2023 V2 edition was released in May 2023. The guidelines are based on the latest research advances and high-level evidence-based medical evidence to establish screening criteria for lung cancer, especially for non-small cell lung cancer, which is the most common and highly regarded type of lung cancer, and has received widespread attention from physicians worldwide. In this article, the latest version of the guideline will be interpreted based on China's national situation and Chinese lung cancer screening guidelines, with the aim of providing an updated reference for lung cancer screening in China.
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Objective To summarize the experience of operation treatment of severe mitral stenosis associated with small left ventricle.Methods The clinical data of 115 patients with severe mitral stenosis received mitral valve replacement (MVR) were retrospectively analyzed.According to whether associated with small left ventricle,they were divided into small left ventricle group (61 cases) and non-small left ventricle group (54 cases).The postoperative early complication rate and mortality rate between two groups were compared.Results The postoperative early complication rate and mortality rate in small left ventricle group was higher than that in non-small left ventricle group [14.75％ (9/61) vs.7.41％ (4/54),55.74％ (34/61) vs.25.93％(14/54)],there was significant difference(P ＜ 0.05).Conclusions The positive and effective perioperative management and correct intraoperative decision is key to reduce the incidence of early postoperative complications and mortality after MVR surgical in patients with severe mitral stenosis associated with small left ventricle.
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OBJECTIVE@#Dihydroartemisinin is a traditional anti-malarial drug, a derivative of the artemisinin, it has anti-tumor activity of a variety of tumor cells. This study investigated the effect of growth inhibition of nasopharyngeal carcinoma cells line CNE-2 induced by dihydroartemisinin and its possible mechanism.@*METHOD@#The effect of DHA on the cell proliferation of CNE-2 was detected by CCK-8 assay with different concentrations and time. The effects of DHA on the cell apoptosis of CNE-2 were detected by Annexin V-FITC assay through flow cytometry and caspase-3 activity assay.@*RESULT@#CCK-8 experimental results show that CNE-2 cell proliferation was suppressed with DHA treatment, as compared with the control group. DHA could induce marked apoptosis in CNE-2 by apoptosis assay, as compared with the control group, The percentages of apoptotic cells increased along with the increase of DHA concentrations in CNE-2; The activity of caspase-3 was increased following DHA treatment in a dose-dependent manner.@*CONCLUSION@#DHA could effectively inhibit proliferation and induce apoptosis of human nasopharyngeal carcinoma cells line CNE-2, the possible mechanism DHA induce apoptosis of CNE-2 cells by upregulating the expression of caspase-3.
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Humans , Apoptosis , Artemisinins , Pharmacology , Carcinoma , Caspase 3 , Metabolism , Cell Line, Tumor , Nasopharyngeal Carcinoma , Nasopharyngeal Neoplasms , Metabolism , PathologyABSTRACT
Objective To investigate the expression and clinical significance of Survivin and Ki-67 in breast carcinoma,and explore their correlation.Method The expression of Survivin and Ki-67 in breast carcinoma(49 cases)and normal breast tissues(12 cases)were examined by immunohistochemical method.Results The positive expression rates of Survivin and Ki-67 in breast carcinoma were significantly higher than those in normal breast tissues(P<0.05).The expression of Survivin was significantly related to clinical stage and lymph node metastasis(P<0.05).The expression of Ki-67 Was significantly related to clinical stage,lymph node metastasis,and pathological grade(P<0.05).The expression of Survivin was obviously related with Ki-67 in breast carcinoma(r=0.734,P<0.05).Conclusions The overexpression of Survivin and Ki-67 may play a cooperative role in the occurrence and development of breast carcinoma.They may serve as useful markers for assessment of biological behavior of breast carcinoma.
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OBJECTIVE@#To study the effects of procaine on human nasopharyngeal carcinoma cell strain CNE-2Z.@*METHOD@#CNE-2Z cells-cultured in vitro-were exposed to different concentration procaine and different time. Its growth characteristic were studied by using MTT assay and flow cytometry. The expression of p16 and RASSF1A mRNA were analyzed by RT-PCR.@*RESULT@#Procaine could inhibit the proliferation of CNE-2Z cells and the effects were in dose- and time-dependent manners. Procaine also arrested CNE-2Z cells in G1 phase of cell cycle. The expression of RASSF1A mRNA was up-regulated in CNE-2Z cells treated with procaine, but no obvious change was found in the expression of p16 mRNA.@*CONCLUSION@#Procaine could inhibit cell growth and up-regulate the expression of RASSF1A mRNA, which may play an important role in CNE-2Z cells' proliferation inhibition induced by procaine.
Subject(s)
Humans , Apoptosis , Cell Line, Tumor , Cell Proliferation , Dose-Response Relationship, Drug , G1 Phase , Gene Expression Regulation, Neoplastic , Nasopharyngeal Neoplasms , Metabolism , Pathology , Procaine , Pharmacology , Tumor Suppressor Proteins , MetabolismABSTRACT
@#Objective To research the relationship between the myocardiac ischemic activity and degree of depression in elderly patients.MethodsThe Center For Epidemiological Studies-Depressive Scale(CES-D) was administered to evaluate depressive degree for 121 patients who were defined as coronary artery diseased by coronary angiography (CAG); group A (n=64) for no depressive symptoms,group B (n=34) for minor depression, group C (n=23) for major depression were studied to observing the myocardial ischemia using Holter's recording and exercise testing.ResultsCAG showed that single vessel disease was more frequently in group A;single,double and three-vessel disease showed approximative proportion in group B,three-vessel disease was more frequently in group C.The times of ST-depress were (4.2±1.3),lasting time of ST-depress was (35.8±9.2) min,and index of ST-depress was (3.28±0.9) min/h,which were higher than those of group A [(1.8±0.3),(16.6±4.2) min,(1.76±0.4) min/h] and group C [(2.1±0.7),(17.8±5.8) min,(1.69±0.5)min/ h](P<0.05);There was significantly frequent myocardial ischemia in group B than in group A during exercise testing (χ2=3.274,P<0.05). patients in group C tended to have approximative incidence of ischemia compared with group A during daily living,but more incidence of ischemia than group B during exercise testing(χ2=5.473,P<0.05).ConclusionThe depressive degree was associated with severity of coronary artery disease,mild to moderate symptoms of depression are associated with an increased likelihood of myocardial ischemia during daily living and exercise,but patients with severity depression absence symptoms of myocardial ischemia during daily living,and exhibit markedly myocardial ischemia during exercise.
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Objective:To detect the changes of tumor necrosis factor-α(TNF-α) in nasopharyngeal secretion of patients with nasopharyngeal carcinoma(NPC).Method: The content of TNF-α in nasopharyngeal secretion was determined by radioimmunoassay (RIA) in 20 controls,52 patients with NPC.Result:The average concentrations of TNF-α were (12.95±4.62)pmol/L in control group,(33.68±15.13) pmol/L in NPC group and (64.00±11.57)pmol/L in NPC with necrosis group. The content of TNF-α in nasopharyngeal secretion in control group was lower significantly than that of patients with NPC (P<0.01).Conclusion:The level of TNF-α in nasopharyngeal secretion of NPC patients was higher than that of normal subjects. To detect the content of TNF-α in nasopharyngeal secretion is a useful test for the study and diagnosis of NPC.
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Objective:To study the effect of airway IL-4RA gene transfer on asthma-associated expression of STAT6 in the mice of allergic asthma.Methods:Forty BALB/c mice were randomly divided into four groups:blank control,asthma model,asthma model administered by pLNC-laz,and asthma model administered by glucocorticoid and pLNC-IL-4RA.The ten mice in each groups were sensitized by i.p.injection of 10 ?g of OVA emulsified in 40 mg of aluminum hydroxide in a total volume of 200 ?l on the days of 1st,7th and 14th.On the 15th day,the mice were challenged via airways with OVA for 7 days,20 min a day,blank control were sensitized by i.p.injection of 200 ?l pH=7.4 PBS on days 7 and 14.Asthma model was administered by pLNC-laz or pLNC-IL-4RA for 3 days beginning at the 12th day.The mice of the group with glucocorticoid was administered with glucocorticoid on the 15th day for 7 days.24 hours after the last challenge,the mice were sacrificed by bloodletting.Serum OVA-specific IgE and blood Eosinophils were measured.The lungs were fixed by immersion in 10% formalin.Expression of STAT6 was identified by immunohistochemistry.Results:It was shown that pLNC-IL-4RA was integrated to the genomic DNA of pneumonic tissues.Retrovirus-mediated delivery of IL-4RA to airways of the asthma mice weakened airway eosinophilia triggered by either IL-13 or IL-4.Furthermore,IL-4RA delivered by retrovirus was expressed in airways of the mice with allergen sensitization,resulting in significiant reduction of expressing level of asthma-associated STAT6 in the experimental mice of allergic asthma.Conclusion:Retrovirus mediated delivery of IL-4RA to airways reduces expression of STAT6 in pneumonic tissues of asthmatic mice.Thus,the gene theraphy can be a potential therapeutic option to treat and control chronic airway inflammation and asthmatic symptoms.