ABSTRACT
IgA plays an important role in the course of Henoch-Schonlein purpura.The current study shows that the HSP is characterized by IgA principally IgA1) depositions in the wall of dermal vessel and the renal mesangium, and IgA1 is deficient in galactose. IgA1 with aberrant glycosylation has a tendency to be self-aggregated, not to be efficiently cleared by the hepatic asialoglycoprotein receptor. It also weakens the ability of complement to remove immune complexes, and causes the exposure of new epitope and so on. All these lead to the formation of IgA1 immune complexes and the deposition in the wall of dermal vessel and the renal mesangium. IgA1 immune complexes activate complement principally through the alternative pathway, which causes inflammatory injury. In addition, various IgA autoantibodies, such as anti-endothelial cell antibodies,anticardiolipin antibodies and anti-neutrophil cytoplasmic antibodies, are also involved in the process of tissue damage of Henoch-Schonlein purpura.
ABSTRACT
Objective To develop a method for simultaneous determination of three kinds of anti-dandruff agents,namely ketoconazole,climbazole,piroctone olamine,in cosmetics by HPLC.Methods The cosmetics were extracted with acetonitrile by ultrasonic,then separated with high-speed centrifuge.The supernatant layer was filtrated through membrane with 0.45 ?m diameter.The filtration liquid was injected into HPLC for detection at ?=210 nm.Quantitative analysis was performed with external standard method.Results In the linear range of 1-250 mg/L,the regression equation of ketoconazole was y=32.23x+ 4.59,the detection limit was 0.069 mg/L.In the linear range of 1-500 mg/L,the regression equation of climbazole was y=13.76x+ 9.41,the detection limit was 0.12 mg/L.In the linear range of 10-500 mg/L,the regression equation of piroctone olamine was y= 24.83x-17.17,the detection limit was 0.71 mg/L.The recoveries and relative standard deviations were 91.8%-98.5% and 0.38%0.84%,respectively.Conclusion The experimental results show that the method is simple,precise and accurate and suitable for simultaneous determination of 3 kinds of anti-dandruff agents in cosmetics.