ABSTRACT
Objective To evaluate the feasibility of speckle-tracking echocardiography in detecting left atrial (LA) function impairment in early hypertensive (HT) patients.Methods Echocardiography was performed in 154 HT patients with LA volume index ＜28 ml/m2 and 64 age/gender-matched control subjects.LA global longitudinal strain in late diastole (Sa),systole (Ss),and total strain (Stot =Sa + Ss),strain rate in late diastole (SRa),systole (SRs),and early diastole (SRe) were measured using off-line speckle-tracking analyzing software in apical 4 chamber view.Results Both parameters reflecting LA reservoir function [Stot (23.7 ± 6.0) ％ vs (25.7 ± 5.9) ％,p =0.03 and SRs ( 1.2 ± 0.3) s- 1 vs ( 1.3 ±0.3) s-1,P =0.03]and conduit function [Ss (12.0 ± 5.1)％ vs (14.0±5.7)％,P =0.02 and SRe (1.0 ± 0.4)s- 1 vs ( 1.2 ± 0.4)s- 1,p ＜0.001 ]decreased significantly in HT group than control,while LA pump function parameters (Sa and SRa) had no differences.Ss,Stot and SRe correlated significantly with HT and HT duration.Conclusions LA phasic function are impaired in HT patients with normal LA size.Speckle tracking echocardiography is a promising tool for the early detection of LA dysfunction.
ABSTRACT
Objective:To investigate the feasibility of detecting excision repair cross-complementing 1(ERCC1)and ERCC2 in peripheral venous blood instead of cancer tissues from esophageal squamous cell carcinoma patients. Methods:The expressions of ERCC1 and ERCC2 mRNA were detected by using RT-PCR in 39 cases of peripheral venous blood samples, esophageal squamous cell carcinoma tissues, and adjacent normal tissues. ELISA was used to determine the levels of ERCC1 and ERCC2 proteins in serum. The periphe-ral blood from 10 healthy volunteers was used as control. Results:Expression levels of ERCC1 and ERCC2 mRNA and protein were significantly higher in peripheral blood from healthy control than those in esophageal carcinoma patients (P<0.05). There was a positive correlation between the expression of ERCC1 and ERCC2 mRNA in peripheral blood and cancer tissues (P<0.01). Conclusion:The expression levels of ERCC1 and ERCC2 mRNA in peripheral blood can indirectly reflect their expression levels in human esophageal squamous cell carcinoma tissues.
ABSTRACT
Objective To study changes in neural stem cell proliferation in ischemic brain tissue after cerebral ischemia/reperfusion injury treated with mild hypothermia. Methods The middle cerebral arteries (MCA) of Sprague-Dawley rats were occluded for 2 hours, then reperfused for 3, 7, 11, 14, 18 and 28 days. Using immunohisto-chemical staining, the bromodeoxyur-idine ( BrdU)-positive cells in the brain tissue of the operationally intervened side were examined in a sham-operation group, a control group, and in rats treated using mild hypothermia. Results There were a few BrdU-positive cells in the sham-operated rats, but there were obviously more in the mild hypothermia group than in the control group. The peak period for proliferation of neural stem cells in the ischemic brain tissue was longer in the mild hypothermia group than that in the control group. Conclusion Mild hypothermia may promote proliferation of neural stem cells in ischemic brain tissue after cerebral ischemia/reperfusion injury.
ABSTRACT
Objective To explore the relationship between the changes of Caspase 3mRNA expression and the injuried nervous cell apoptosis after rat focal cerebral ischemia/reperfusion injury who was then treated by mild hypothermia.Methods The middle cerebral arteries(MCA) of SD rats were occluded for 2 hours,and reperfused for 12 hours.Using FCM and semiquantitative RT-PCR technique,the DNA fragmentation rate and Caspase 3mRNA expression level were detected in the shamoperation group,the control group and the mild hypothermia group,respectively.Results The DNA fragmentaation rate and Caspase 3mRNA expression level in the sham operation group and the mild hypothermia group were obviously lower than those in the control group.Conclusions The significantly decreased level of Caspase-3mRNA expression may be related to the obviously decreased nervous cell apoptosis after rat cerebrall ischemia/reperfusion injury treated by the mild hypothemia.
ABSTRACT
Objective To study the relationship between Bcl-2/Bax protein expression and the degree of malignancy of astrocytoma.Methods Bcl-2/Bax protein expression positive rate and apoptotic cell rate were determined using immunohistochemical technique and TUNEL, in 10 normal human brain tissues and 80 human astrocytoma specimens of differently malignant degrees confirmed by pathology separatively. Results Bcl-2/Bax protein positive expression was not found in normal human brain tissues, and apoptotic cell rate was only (0 6?0 1)%. The differences of Bcl-2/Bax protein expression positive rate and apoptotic cell rate were statistically significant in the differently malignant degrees(P
ABSTRACT
Objective: To design and confirm the cleavage activity of ribozyme Rz217 to oncogene ki-rasG12V messenger RNA and search for a new method for gene therapy targeting oncogene ki-ras. Methods: According to Symon' s principle,design an ribozyme specific for ki-rasc12v mRNA, both the constructs for transcription in vitro of ribozyme Rz217 and ki-ras exonl and the mammalian expression constructs of ribozyme Rz217 were constructed by DNA recombinant technique,ribozyme Rz217 and ki-ras exonl mRNA was obtained by transcription in vitro with T7 and SP6 RNA polymerase. Pancre atic carcinoma cell line PaTu8988 and human hepatocellular carcinomacell line BEL7404 were transfected with Rz217 mammalian expression constructs and the level of endogenous ki-rasG12V mRNA or ki-ras mRNA was determined by semiquantitative RT-PCR. Results: Not only in vitro but also in vivo, ribozyme Rz217 can cleave the mRNA of oncogene ki-ras (G12V) in site-specific manner and can not cleave the mRNA of wild-type ki-ras. Conclusion: Ribozyme Rz217 can cleave oncogene ki-rasc12v mRNA and the cleveage is specific for ki-rasG12V mRNA.