ABSTRACT
Objective: To investigate the expression level and clinical significance of a long non-coding RNA (LncRNA), BC200 in non-small cell lung cancer (NSCLC) and analyze its correlation with the epithelial-mesenchymal transition (EMT)-related proteins, E-cad-herin, N-cadherin, and Snail. Methods: Sixty NSCLC and sixty paired adjacent tissue samples were collected to detect the BC200 levels. Furthermore, 40 samples of NSCLC and 40 samples of normal lung tissues were collected to quantify the messenger RNA (mRNA) lev-els of E-cadherin, N-cadherin, and Snail using quantitative polymerase chain reaction. The relationship between the BC200 level and mRNA levels of E-cadherin, N-cadherin, and Snail was explored in NSCLC tissues. The correlation between BC200 and clinical pathologi-cal parameters (gender, age, TNM stage, tumor size, lymph node metastasis, and pathologic type) was also analyzed. Receiver operat-ing characteristic curve (ROC) was constructed to evaluate the diagnostic efficiency of BC200. Immunohistochemical staining was per-formed to determine the expression levels of E-cadherin, N-cadherin, and Snail in 40 specimens of NSCLC and 20 specimens of normal lung tissue and their correlation to the expression levels of BC200 was evaluated. Results: 1) The expression of BC200, N-cadherin mRNA, and Snail mRNA was significantly upregulated in the tumor tissues when compared to that in normal lung tissues (P<0.05). The expression of E-cadherin mRNA was significantly lower in tumor tissues than in the normal lung tissues (P<0.05). 2) The positive rate of E-cadherin, N-cadherin, and Snail in NSCLC was 40% (16/40) , 57.5% (23/40), and 57.5% (23/40), respectively, while that of normal lung tissues was 95% (19/20), 5% (1/20), and 10% (2/20), respectively. There was a significant difference between these two data sets (P<0.05). 3) BC200 is highly expressed in the NSCLC tissues. The high expression of BC200 in lung cancer was correlated with lymph node metastasis, clinical stage, and positive rate of E-cadherin, N-cadherin, and Snail. The expression of BC200 in NSCLC tissues was negatively correlated with the expression of E-cadherin mRNA (r=-0.31, P<0.05) and positively correlated with the expression of Snail and N-cadherin mRNA (r=0.305, r=0.257, P<0.05). 4) ROC analysis of BC200 indicated a potential diagnostic value of BC200 levels in NSCLC . Conclusions: BC200 is highly expressed in NSCLC tissues, which was correlated with lymph node metastasis, clinical stage, E-cadherin, N-cadherin, and Snail positive rate. The expression of BC200 in NSCLC tissues was negatively correlated with E-cadherin and positively correlated with Snail and N-cadherin. BC200 may regulate the invasion and migration ability of NSCLC by EMT. BC200 may be a potential tumor marker for NSCLC diagnosis.
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Objective To study the effect of Danggui-beimu-kushen Pill on benign prostatic hyperplasia (BPH) and its possible mechanism. Methods BPH model mouse was produced by intraperitoneal injection of testosterone propionate. The high, medium and low dose treatment group was fed high, middle and low dose of Danggui-beimu-kushen Pill respectively, while the positive control group was fed Qianliekang liquid, 600 mg/kg. All mice were executed on the 21 day. Such values were observed as the prostate index changes, pathological changes of prostate by HE staining light microscopy and the expression changes of PCNA,bcl-2 and bax by immunohistochemistry. Results The prostate index of the treatment group was lower than the model group; the PCNA and bcl-2 expression were lower than the model group, while the bax expression was higher than the model group. Meanwhile; there is no significant difference among the treatment groups.Moreover, the difference between the treatment groups and the positive control group has no statistic meaning.Conclusion Danggui-beimu-kushen Pill can suppress BPH in mice. The mechanism may be related to the reduction expression of cell-proliferation protein PCNA and apoptosis profilin bcl-2, and the increase of bax expression.
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OBJECTIVE: To study the effect of samples having different pH values in the bacterial endotoxins test (BET). METHODS: The BET was conducted as an assay of the concentration of endotoxins by using kinetic-turbidimetric technique. Samples having different pH values but containing same concentration reference standard endotoxin (RSE) were tested by TAL/LAL reagents. The average result on the standard curve was calculated and the mean recovery observed. RESULTS: The reagents had three highly sensitivities in pH 5.20, pH 7.83/pH 6.23 and pH 10.55. After adjustment to pH 6 to 8, the mean recovery was 76.5%～115.9% or 85.8%～99.8%. When the sample pH value was less than 3 or more than 12, the test was unsuitable for the inhibition test, and the mean recovery was less than 56.8%. CONCLUSION: It is necessary to adjust the pH of the solution to be tested in the BET, adjust the sample to pH (6.5～7.5) when using TAL reagents, and adjust to pH (6.2±0.1) or pH(6.7～7.8) when using LAL reagents.