ABSTRACT
Objective To systematically assess the diagnostic efficacy of circRNA in detecting CRC. Methods Eligible studies were obtained by searching the databases, such as PubMed, EMBASE, CNKI, etc. in line with the inclusion and exclusion criteria. Study quality and bias were judged by QUADAS-2 tool. The overall effect size, including pooled diagnostic parameters, was combined based on STATA 12.0 software. The causes of heterogeneity were investigated by conducting the subgroup, sensitivity and meta-regression analyses. Results We included 17 eligible studies which contained 1873 CRC cases and 1573 paired controls. For CRC detection, circRNA expression harbored a pooled sensitivity, specificity and AUC of 0.79 (95%CI: 0.76-0.81), 0.69 (95%CI: 0.64-0.73) and 0.81, respectively. Subgroup analysis showed that serum-derived circRNA testing (AUC=0.89, DOR=16.79) harbored higher diagnostic efficacy than plasma (AUC=0.79, DOR=7.08) and tissue (AUC=0.76, DOR=6.87); oncogenic circRNAs achieved higher diagnostic capacity in detecting CRC than the anti-cancer circRNAs (AUC: 0.81 vs. 0.76; DOR: 8.54 vs. 6.21); however, circRNA testing quantified based on GAPDH showed comparable diagnostic capacity with the overall pooled effect. Conclusion Abnormally-expressed circRNAs have relatively high diagnostic efficacy in diagnosing CRC and may be rated as auxiliary biomarkers for CRC detection.
ABSTRACT
Objective To explore the relationship between gene MTDH expression and its role in promo-ting gastric carcinoma metastasis .Methods We collected clinical specimens and cultured gastric carcinoma cell lines.By Western blotting and Real -time PCR methods,protein and mRNA levels in tissues and MTDH relation-ship with EMT were detected .Results There was 86%of patients who expressed MTDH positively and 13%of normal gastric mucosa was positive expression .The results showed that the expressive level of MTDH gene in gas-tric carcinoma was higher than in the normal tissues .The expression of MTDH was correlated with TNM stage、mi-crovascular invasion、recurrence and metastasis .The expressive level of MTDH was correlated with two epithelial mesenchymal transition markers ( E-cadherin and N-cadherin ) .Conclusion MTDH may promote gastric car-cinoma metastasis through the induction of EMT process and may be a candidate biomarker for therapeutic target .
ABSTRACT
Objective To evaluate the therapeutic effect of pulmonary carcinoma by bronchical arterial infusion (BAI) and bronchical arterial infusion with the addition of pulmonary arterial infusion (PAI).Methods Totally 69 cases with pulmonany cancinoma were treated by intervention therapy .Of them ,38 cases were undergone the bronchical arteriography and arterial infusion and 31 cases were undergone the brochical pulmonany arterial dual infusion.Results The bronchical arteryies taken part in the blood supply of neoplasmin all cases ,while the pulmonany artery only in 77% of cases .The effective ratio of BAI +PAI was 87%, and BAI was 65.7%,there were markly different (?