Ethanol affects the absorption and tissue distribution of orally administered antigens in mice

The aim of this work was to evaluate the effects of ethanol on the adsorption and tissue distribution of orally administered antigens in mice. Results showed that ethanol reduced the level of anti-ovalbumin IgA antibodies in intestinal fluid for the mice treated orally with a palmitoyl-ovalbumin conjugate. Ethanol was administered intragastrically to mice at 5 g/kg body weight for 14 days (chronic treatment), or 10 g/kg body weight every 7th day up to 14 days (acute treatment). Thereafter, 99m technetium-labeled antigens were administered and lymphoid tissues were collected. Ethanol interfered with the transport of ovalbumin to the liver. Moreover, the transport of palmitoyl-ovalbumin to mesenteric lymph nodes was reduced 6 h after the antigen administration. In conclusion, there was a relationship between the suppression of ethanol-mediated specific local IgA responses and the decreased transport of palmitoyl-ovalbumin to mesenteric lymph nodes.

Modulação da inflamação por ômega-3/6 / Modulation of the inflammation caused by Omega -3/6

Vários estudos vêm demonstrando os efeitos benéficos dos ácidos graxos poliinsaturados, especialmente do tipo ômega-3, no processo inflamatório. Esses lipídios, por meio de interferências na regulação gênica e no metabolismo celular, desempenham um papel modulador das reações in amatórias agudas ou crônicas. Neste trabalho, utilizou-se o modelo inflamatório induzido por implantes subcutâneos de parafina afim de avaliar os efeitos da administração dos ácidos graxos poliinsaturados por via oral e subcutânea. Foram utilizados camundongos Swiss, fêmeas, com 6 a 8 semanas de idade. Posteriormente, os animais receberam um implante subcutâneo de tabletes de parafina afim de induzir um processo inflamatório crônico. Vinte e um dias após a indução da inflamação, os animais receberam 100μl/camundongo de óleo de peixe contendo 180mg de ácido eicosapentaenóico e 120mg de ácido docosahexaenóico via oral. Pela via subcutânea, foram inoculados 20μl/camundongo de óleo de peixe contendo 3,6mg de ácido eicosapentaenóico e 2,4mg de ácido docosahexaenóico. Os camundongos receberam 8 (1 vez/semana) ou 24 (3 vezes/semana) inoculações, dependendo do experimento. O grupo controle (n = 5) recebeu tratamento com salina...

Several studies show that omega-3 polyunsaturated fatty acids suppress the immune response and inflammation. Some of the effects of omega-3 are brought about by modulation of the amount and types of eicosanoids made, and other effects are elicited by eicosanoid-in dependent mechanisms, including actions up on intracellular signaling pathways, transcription factor activity and gene expression. The aim of this study was to evaluate the effect of oral or subcutaneous omega-3 administration in chronic pathological conditions advancing.To determine whether chronic inflammation induced by paraffin implants is altered by w-3 supplementation, six to eight-week-old male Swiss (n = 5)...