Detection of Human Papillomavirus Type 16 and 18 in Cervical Specimens by Nested Polymerase Chain Reaction / 대한임상병리학회지

BACKGROUND: Some types of human papillomavirus (HPV) play a major role in the pathogenesis of cervical cancer. Several dozens of studies on the association of HPV with cervical neoplasm have been done since the first detection of HPV 16 and 18 directly from cervical cancer patients in 1983. Approximately 90 types of HPV have been identified so far and the number of oncogenic HPV types is still growing. In this study, we examined the occurrence of oncogenic HPV infections in patients with cervical lesions. Method : Two hundred twenty cervical swab specimens were collected during a 3 year period (1996-1999). Processed specimens were tested for HPV type 16 and 18 by polymerase chain reaction. RESULTS: HPV type 16 was detected in the cervical swab specimens as follows: 19 (51.4%) of 37 cervical cancer patients, 19 (30.2%) of 63 high-grade squamous intraepithelial lesions, 5 (9.6%) of 52 low-grade squamous intraepithelial lesion, none in 6 atypical squamous cells of undetermined significance and 3 (4.8%) of 62 normal cervices. Conclusion : The positive rate for HPV type 16 increased according to the degree of cervical malignancy.

Ultrasonography and ultrasonoguided percutaneous fine needle aspiration biopsy of pancreatic cancer

Ultrasonograhy of pancreas has been already established to be a effective method in the evaluation ofpancreatic mass lesion. In addition to ultrasonography, ultrasonoguied percutaneous fine needle aspiration biopsyof pancreatic mass has been known to be a safe method of obtaining tissue diagnosis in patients with pancreaticcancer without operation. From March, 1984 to June, 1986 ultrasonography and ultrasonoguided percutaneous fineneedle aspiration biopsy were peformed in 40 patients who had been finally diagnosed as pancreatic cancer at theDepartment of Radiology, Yonsei University College of Medicine. The results were summarized as follows: Inultrasonographic findings of pancreatic cancer, (1) The location of pancreatic mass in 19 cases in head, 3 in headand body, 7 in body, 4 in body and tail, 5 in tail and 2 diffuse type. (2) The size of tumor ranged under 2cm in 2cases, between 2.1–4cm in 21, between 4.1–6cm in 13 and over 6cm in 4 cases. (3) The other ultrasonographicfindings were 6 cases common bile duct dilatation, 4 pancreatic duct dilatation, 10 peripancreatic nodeenlargements, 16 liver metastatsis and 3 stomach involvement. In ultrsonoguided percutaneous fine needleaspiration biopsy of total 40 cases of pancreatic cancer. (4) The cytologic diagnosis were postive malignant cellin 31 cases( 77.5%), highly suspicious malignant cell, but inconclusive in 3(7.5%), negative malignant cell in 2(5.0%) and material insufficiency in 4 cases(10%) (5) No significant complication durinng or after procedure wasfound.