ABSTRACT
Background: Salvia multicaulis Vahl. a medicinal plant belonging to the Lamiaceae family, has an extensive application in native and traditional medicine
Objective: This research was conducted to investigate diversity of morphophysiological traits and content of essential oil, phenol and flavonoid of Salvia multicaulis ecotypes in different districts of Hamedan province, Iran
Methods: In this study, 11 ecotypes of Salvia multicaulis were collected from different districts of Hamedan Province at the full flowering stage in spring 2016 and were evaluated for their morphophysiological and phytochemical characteristics. The classification of ecotypes was done on the basis of phytochemical and morphophysiological traits by cluster analysis and correlations among quantitative traits was also conducted by Pearson method
Results: Result showed that the essential oil content had positive significant correlation with plant height, inflorescence length, dry matter of flowering branche and essential oil yield. Also there was a positive significant correlation between essential oil yield with receptacle length, flower fresh and dry matter and plant dry matter. The heighes dry weight of flowering branch and flower was related to ecotypes of Lashkardar and west of Hamedan, respectively. The maximum plant dry weight was belong to Yelfan ecotype. The most essential oil content was related to Lashkardar and west of Hamedan ecotypes. Also, the highest content of phenol and flavonoid related to Vehnan ecotype. According to the cluster analysis, 11 ecotypes were divided into two groups
Conclusion: The evaluation of morphophysiological and phytochemical traits showed that there was a considerable variation among different ecotypes of Salvia multicaulis in respect of morphophysiological and phytochemical characteristics
ABSTRACT
Background: One of the new methods to overcome problems with using essential oils [such as instability, evaporation and decomposition in the environmental conditions[ in pharmaceutical, food and agricultural industries is use of Solid Lipid Nanoparticles [SLNs] carrier systems
Objective: Preparation of SLNs containing the Zataria multifloni Boiss essential oil. Methods: In this regard, an experiment was performed on the preparation of SLNs containing the Zataria multiflora Boiss essential oil at Nanotechnology Research Center of School of Pharmacy, Mashhad University of Medical Sciences. SLNs containing Zataria multiflora Boiss essential oil was prepared using high tensile pressure homogenization and ultrasound
Components of SLNs include lipids, Zataria multiflora Boiss essential in fat phase and poloxamers 188 in the aqueous phase
The mean particle size and zeta potential, essential oil encapsulation percentage and thermal analysis were measured using particle size analysis instruments, gas chromatography and differential scanning calorimetry [DSC], respectively. Moreover, an electron microscope was used for imaging of SLNs
Results: The results showed that particle size, polydispersity index and zeta potential of the above formulations were respectively about 486 nm, 0.296, -27.2 mv. The results obtained from transmission electron microscopy [TEM] showed particle size less than 300 nm and particles were spherical
Thermal analysis by DSC, confirmed the presence of solid particles in the prepared SLN. Also, the essential oil encapsulation percentage was 95.2 percent. Stability studies of particle size and zeta in four months showed SLNs containing essential oils had relatively good stability
Conclusion: In general, the results of the present research showed that SLNs composed of stearic acid, was good carriers for Zataria multifloni Boiss essential oil
ABSTRACT
Domestic cultivation under natural habitat may help to genetic diversity conservation and sustainable utilization of medicinal plant. Since alkaloids are nitrogenous compounds, the availability of nitrogen is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants. The aim of this research was to study the effect of bio-fertilizer and chemical fertilizer on corm and colchicine yield in Colchicum kotschyi under natural habitat. The experiment was performed in a randomized complete block design with eight treatments including of three levels of urea as nitrogen fertilizer [50, 100 and 150 kg ha[-1]], three levels of Nitroxin [20, 40 and 60 L ha[-1]], combined treatment [urea 100 kg ha[-1] + Nitroxin 40 L ha[-1]] and no fertilizer as control with three replications at the natural habitat, during 2009 -2010. results showed that the application of Nitroxin 40 L ha[-1]resulted in the highest corm dry yield [101.4 g], while the lowest corm dry yield was obtained in control [42.39 g]. The yield of colchicin was higher in application of urea 100 kg ha[-1] [40.44 mg] or Nitroxin 40 L ha[-1] [37.58] than to other treatments, while the lowest colchicine yield was obtained in control [15.56 mg]. Generally moderate levels of chemical and biological fertilizers [urea 100 kg ha[-1]or Nitroxin 40 L ha[-1]] had the best results on corm and colchicine yield than to other treatments