Aminophylline LD50 measurement at male mouse

Aminophylline is a bronchodilator used in the management of the reversible airway obstruction due to asthma, chronic bronchitis, and emphysema. The incidence of toxicosis due to the treatment by Aminophylline has a remarkable frequency, and a great importance especially in elderly patients, so that the study has carried out to determine the lethal dose of 50% [LD 50%] of mice after inject them in the peritoneum by increasing doses of Aminophylline. The study has been shown that Aminophylline has an acute toxic effect which appears during the first hour

Induced mutants of Streptococcus lactis by gamma irradiation and its effect on milk acidity

Streptococcus lectic was exposed to different doses of gamma- irradiation [10, 50, 100 and 150 Kr]. The results of acidity production in sterilized milk inoculated by isolates from radiation treatments and control could be summarized in the following: The mean acidity produced by S. lactic isolates after irradiation at 10 Kr increased to be 0.66% than that of control isolates [0.62%]. The acidity produced by the isolates of the 50 Kr treatment showed more increment to reach the peak [0.7%]. Thereafter, acidity production decreased by isolates of each 100 Kr [0.53%] and 150 Kr [0.51%] treatments. Hence, the 50 Kr treatment could be considered activation dose to S. Lactic starter for acid production. Two mutants were selected. Acidity production by mutant I [from 10 Kr treatment] was 0.95%, and that of mutant II [from 50 Kr treatment] was 1.0%, while acid production by the parent S. lactic culture was 0.62%. Concerning the stability of isolates, it was found that the acid production by mutant I and mutant II slightly decreased by time. The mutants were re-irradiated after 37 and 60 days at doses 10, 25 and 50 Kr. Acid production in milk by isolates of the radiation treatments was determined