ABSTRACT
H.pylori is a microaerophilic, garm-negative, spiral organism with 4-6 flagella. The bacterium is an etiologic agent for chronic gastritis and plays a major role in effecting peptic ulcer, gastric cancer, lymphoma, H. pylori infection has a high seroprevalence in iran [> 90%], thus, diagnosis and treatment of H. pylori infection is very important. The presence of H. pylori infection was determined in 100 patients undergoing upper gastrointestinal endoscopy over 9 month period. The presence of H. pylori was detected in gastric mucosal biopsy specimens through Biopsy Urease Test, PCR and histopathology. A medium was used for urease test, Normal Broth Urea. The biopsy is placed in a both containing urea and a PH indicator. The gold standard is histopathology examination. We designed a PCR for amplifying the H. pylori gene encoding ure C [294bp]. The results of urease test were 66.6% sensitivity and 69.7% specefity. The results of PCR assay were 98.6% sensitivity and 93% specifity. There was a significant difference between the specifity and sensitivity of urease test and PCR. The result of this study showed that urease test was not a sensitive and specific method for accurate detection of the infection, level of the sensitivity is too low to be used as the sole method of detection in ordinary practices. In this situation, other tests should be used
ABSTRACT
The present study was conducted to determine the efficacy of cryoanalgesia and its complications in patients with post-thoracotomy pain, operated in Masih-Daneshvari hospital in 2001-2.Sixty patients entered this clinical trial. After thoracotomy, patients were equally distributed in two groups of control and cryoanalgesia. Nerve freezing was carried out for 60 seconds at a temperature of -60 degrees Celsius. Pain was measured by Visual Analogue Scale. Meanwhile, patient's demand for pethidine was recorded till the 7[th] day. Sensory complications were surveyed through telephone call till the 3[rd] postoperative month. Pain score, sensory complications and the amount of pethidine administration were compared between the groups. The mean age [ +/- SD] of control and "Cryo" patients were 41.3 +/- 15 and 41.9 +/- 16 years, respectively. In the recovery room, pain score of 2-3 was reported in 63.3% of control subjects, the remaining 36.7% had experienced pain score of 4-5. These figures were 86.7% and 13.3% for the "Cryo" group, respectively [p < 0.05]. During the following days, pain score was significantly higher in the control subjects. At the 7[th] day, however, pain score of 0-1 and 2-3 were reported in 13.3 and 86.7% of the control subjects, whereas these figures were 83.3 and 16.7% for the "Cryo" subjects, respectively [p < 0.00001]. During the first day, pethidine was administered 151.6 +/- 27 mg and 87.5 +/- 48 mg for the control and "Cryo" subjects, respectively [p < 0.001]. Hypoalgesia, reported in 93.3% of the patients at the end of the first week, disappeared by the end of the 3[rd] month. Allodynia and dysesthesia was reported in 10% of the subjects at the end of the first week and vanished by the second month. Cryoanalgesia could safely diminish post thoracotomy pain; also, it may eliminate the need for opiates. It has few complications that are soon relieved. We strongly recommend this technique and complementary research in this field