[Prevalence of obesity, abdominal obesity and hypertension in 10-13 years old children of governmental and non-governmental elementary school in some regions of Tehran in 1390 year]

Obesity and hypertension disorders affect whole body and are associated with most diseases, including diabetes and cardiovascular disease. Children with obesity and high blood pressure are prone to adulthood health problems. Yet most parents are unaware of their child obesity and high blood pressure. This 2011 study, included 1184 boys and girls aged 10-13 years old from five districts of Tehran. Overweight and obesity were diagnosed by the International Obesity Task Force [IOTF] criteria. Also, systolic or diastolic blood pressure >/= 95 for age, height and gender were considered as hypertensive. Waist circumference according to the standard Iranian cut off for children was assessed on the basis of age, gender. Waist circumference > 90 percentile was considered as abdominal obesity. Prevalences of overweight, obesity, hypertension and abdominal obesity were 21.92, 5.28, 13.16 and 25.31% respectively. The highest prevalence of obesity and abdominal obesity reported in District 3, which is north of Tehran and the lowest was related to boys from region 19 of southern Tehran. Economic conditions can affect obesity and hypertension disorders and more attention must be paid to obesity and hypertension in particular, to creat awarences in parents for changes in lifestyle, nutrition and physical activity these children

Recombinant outer capsid glycoprotein [VP7] of rotavirus expressed in insect cells induces neutralizing antibodies in rabbits

Rotaviruses cause diarrhea in infants and young children worldwide. Rotavirus outer capsid protein, VP7 is major neutralizing antigen that is important component of subunit vaccine to prevent rotavirus infection. Many efforts have been done to produce recombinant VP7 that maintain native characteristics. We used baculovirus expression system to produce rotavirus VP7 protein and to study its immunogenicity. Simian rotavirus SA11 full-length VP7 ORF was cloned into a cloning plasmid and then the cloned gene was inserted into the linear DNA of baculovirus Autographa californica Nuclear Polyhedrosis Virus [AcNPV] downstream of the polyhedrin promoter by in vitro recombination reactions. The expressed VP7 in the insect cells was recognized by rabbit hyperimmune serum raised against SA11 rotavirus by Immunofluorescence and western blotting assays. Rabbits were immunized subcutaneously by cell extracts expressing VP7 protein. Reactivity with anti-rotavirus antibody suggested that expressed VP7 protein had native antigenic determinants. Injection of recombinant VP7 in rabbits elicited the production of serum antibodies, which were able to recognize VP7 protein from SA11 rotavirus by Western blotting test and neutralized SA11 rotavirus in cell culture. Recombinant outer capsid glycoprotein [VP7] of rotavirus expressed in insect cells induces neutralizing antibodies in rabbits and may be a candidate of rotavirus vaccine

Prevalence of GBV-C RNA in HIV infected individuals in Tehran, Iran

GB virus C [GBV-C] is a flavivirus that was characterized in 1995. The prevalence of GBV-C RNA in HIV-infected subjects has not been previously studied in Iran and was therefore determined. We have investigated serum samples of 80 patients from Imam Khomeini Hospital, Tehran, Iran, whose HIV infection was confirmed in our laboratory by Western-blotting. We used nested-PCR to detect GBV-C/HGV RNA in their sera. GBV-C/HGV RNA was detected in 15 [18.8%] of 80 patients. There was no significant difference in GBV-C/HGV RNA prevalence between males and females, in different age groups and HIV viral loads groups. The prevalence of GBV-C RNA was high in HIV-infected patients. There was no association between GBV-C RNA prevalence and specific gender, age, and HIV viral loads groups

Molecular analysis of three iranian isolates belonged to 793/B serotype of infectious bronchitis viruses

Infectious bronchitis [IB] disease is one of the important respiratory diseases of poultry that causes annually large economic losses in poultry industry of Iran. The aim of this study is molecular characterization of S1 gene of Iranian infectious bronchitis viruse [IBV] belonged to 793/B serotype. The whole S1 gene of three local IBV strains in RT-PCR, showed a band above 1600 base pair [bp] in gel electrophoresis. RFLP analysis using 3 restriction enzymes, Hae3, Hind3 and Ecor1, showed 793/B serotype pattern. The S1 gene of these strains were sequenced and compared with 30 reference IBV strains. Identity Plot [IP] of nucleic acids and amino acids sequences were also designed. Moreover, nucleic acids differences in 1659 bp of S1 gene were calculated by distance method: nucleotide: Kimura 2-parameter and finally, the phylogenetic tree of S1 gene sequence strains with the highest validity in branching were designed. Three Iranian strains belonged to 793/B genotype with nucleotide differences of 5.64-6.07% to UK/793/B as a prototype of 793/B and 26.02-26.16% to H120 as a vaccinal strain. Regarding to low homology and weak cross protection between 793/B serotype and Massachusetts vaccinal strain, it would be a possible cause for failure in vaccination and outbreak of IB disease in vaccinated flock of poultry industry

Protein pattern of penicillium species isolated from natural sources in Iran

Farctionation of diffecent penicillium species based on protein bands. In this study penicillium citrinum, penicillium oxalicum, penicillium notatum and penicillium frequentes isolated from air in Iran have been compared for their protein pattern antigens. First, the isolates were cultured on sabouraud dextrose agar medium and then subcultured on czapex agar and were maintained on 30°C for 48-72h. Then they were cultured on sobouraud broth medium for preparing protein extracts, and braudford method was used for measuring the level of protein. The proteins were differentiated using SDS-PAGE with 10% separating gel. Coomassie blue G250 was used for staining. 34 protein band with molecular weight of: 19.5, 24, 26, 27, 28.5, 32, 36, 39, 45, 48, 50, 52, 53, 55, 56.5, 59.5, 63, 65, 66.5, 68, 76, 84, 88, 90, 92, 93, 94, 95, 97, 107, 116, 123, 128 and 158 kD were observed. The bands 19.5, 24, 28.5, 45, 52, 53, 56.5, 59.5, 76, 84 and 97kD were present in all 30 isolates under study. The results, indicate that there are inter species and intra species differences but there is no significant difference in protein patterns of the isolates

[Isolation and identification of infectious bronchitis viruses poultry farms of Iran]

Objective: detection of infectious bronchitis viruses by RT- PCR/RFLPs in poultry farms of Iran

Design: longitudinal study from 1997 to 2003

Samples: tracheas, lungs and kidneys of suspected flocks to respiratory diseases

Procedure: from 1997-2003, tissues samples including lung, trachea and kidney, had been prepared from broiler and layer flocks were submitted to avian virology laboratory of poultry diseases section in order to isolate respiratory viral diseases viruses. A total number of 50 infectious bronchitis viruses [IBV] were isolated in embryonated chicken eggs. The infected embryos displayed stunting, urate deposition in the mesonephros or death after three passages. Twelve isolates that had showed typical signs in embryos, were selected for molecular identification. Viral RNA was extracted by RNxTM plus [CinnaGen Co.] using chloroform 1 Isoamyl alcohol, Isopropanol, Ethanol and DEPC water. cDNA was prepared from extracted RNA with RT enzyme, RH primer, RT buffer, dNTP and Rnase inhibitor. For PCR reaction, buffer PCR ]OX, MgC12, S loligo5' and 3' primer, ampli Taq DNA polymerase and dNTP were added to cDNA and then PCR was conducted in thermal cycler. The PCR products were analyzed on a 1% agarous gel and Ethidurn Bromide staining. The S1 glycoprotein genes of IBV strains appeared to be above 1600 bp in size. PCR products were digested by HaeIII, EcorI and HindIII, according to the manufacture's recommendation

Results: base on RFLPs patterns and comparison with RFLP references patterns [793A3, D274, M41, H120], 8 of 12 strains showed 793/B pattern and the rests [4 of 12] showed Mass patterns in RFLPs

Clinical implications: regarding to low homology and weak cross protection between 793B serotype and vaccinal strain [Massachusetts] prevention and a controlled strategy against IB should be altered