[Collaboration coefficient of researchers of Tehran university of medical sciences in international publications]

Introduction: Collaboration between researchers at domestic and international level is an extensive form of scientific collaboration emphasizing the importance and benefits of collaborative research. This study was aimed at investigating the rate of collaboration between researchers in Tehran University of Medical Sciences with their counterparts in other countries and institutions in the Web of Science publications

Methods: This was a survey using scientometrics indicators. The instrument of the study was the Web of Science in which the scientific products of Tehran University of Medical Sciences including 5781 records were investigated between 2006 and 2010.To determine the collaboration between researchers, collaboration coefficient formula was used

Results: The authors' collaboration coefficient mean was 0.64 showing their high interest in collaborative research. Investigation into scientific products showed that the rate of scientific collaboration was lower at the international level [22%] than the domestic level. The results showed that the researchers in Tehran University of Medical Sciences had scientific collaboration with 69 countries. The lowest annual growth rate [3.7] was related to international collaboration. Main partners of Tehran University of Medical Sciences were the USA, UK, and Canada, respectively. Based on the number of co-authors, the highest collaboration rate belonged to the documents with five to eight authors. This rate increased from 33% in 2006 to 45% in 2010

Conclusion: The findings indicated that co-authorship and collaboration in the period studied is on the rise for Tehran University of Medical Sciences suggesting researchers' interest in scientific collaboration

[ survey of knowledge production of Iranian researchers on AIDS: evidence from the web of science database]

The main purpose of this study was to investigate the knowledge productions of Iranian researchers who had published papers on AIDS in journals and sources with impact factor indexed in the Web of Science between 2000 and 2010. The amount of scientific publications by Iranian researchers on AIDS in the Web of Science was studied with scientometric methods. 336 documents with at least one author affiliated to Iranian organizations were identified. The process of science production of Iranian researchers enjoyed an increasing annual growth rate of 30%. Iranian researchers had scientific collaboration with 22 international authors dominated by American authors. Tehran University of Medical Sciences contributed to almost one fifth of the scientific productions among national organizations. Most research studies conducted by Iranian researchers on AIDS were listed under the category of infectious diseases. 35% of the journals in which Iranian researchers published their papers were American journals. However, the journal, Hepatitis Monthly, published the highest number of papers by Iranian researchers. Iran's share of journal publications enjoyed an upward trend internationally, and in this regard, Iranian researchers have remarkable contribution to international joint venture articles

[Detection of viability and metabolic activity of NB4 cell line treated with AZT]

APL is a Prevalent leukemia that Approximately included 5-10% of patients with acute myeloblastic leukemia. ATRA and recently arsenic is used for treatment. ATRA leads to resistance to treatment and arsenic is toxic in high doses. AZT induce cell death in different ways. The purpose of this study was Assessment of effect of AZT, a telomerase inhibitor, on NB4 cell line [APL cell line] to reduce toxic effect of high dose arsenic. In this study, viability and metabolic activity of NB4 cells, treated by different concentrations of AZT[50,100,200 micro M], was assessed by trypan blue dye method and MTT assay respectively. Treated cells with AZT=50,100,200 micro M showed decreased viability, both in dose-dependent and time-dependent through trypan blue dye method and decreased cell metabolic activity by MTT assay. Considering that AZT is able to induce apoptosis and decrease cell activity, it seems AZT is a suitable drug for inhibiting the growth of tumor cells

[Combination effect of low dose of arsenic trioxide and AZT on viability and metabolic activity of NB4 cell line]

Acute promyelocytic leukemia [APL] is a distinct subtype of acute myeloid leukemia. APL is characterized by a balanced reciprocal translocation between chromosomes 15 and 17, t[5;17]]. Important therapeutic strategies for this disease are ATRA and Arsenic trioxide. To eliminate tumor cells with arsenic, high dose of arsenic is needed. But high dose is toxic for normal tissue. The purpose of this study is Assessment of effect of low dose of arsenic trioxide in combination with AZT on NB4 cell line [APL cell line] to reduce toxic effect of high dose arsenic. In this study after NB4 cell line culture and proliferation, the cells treated with low dose of arsenic trioxide[0.5 micro M] in combination with different doses of AZT[50, 100, 200 micro M] and then viability and metabolic activity was assessed by try pan blue and MTT assay respectively. Low dose of arsenic [0.5 micro m] alone and in combination with dose of 50 micro m of AZT has little effect on viability and metabolic activity but in combination with higher dose of AZT has significant effect on viability and metabolic activity and both viability and metabolic activity significantly reduced. Different apoptosis- induced mechanisms cause apoptosis by arsenic and AZT. Since some of these mechanisms between AZT and arsenic are similar, so maybe these similar mechanisms cause synergic effect and significant reduction of viability and metabolic activity in combination of these two drugs

[ effect of different doses of water green tea extract on the development of open wounds in mice]

This research has been done with awareness of positive effects of green tea, and because of the importance of treatment of opened skin wound. The effect of different doses of water green tea extract on the development of open wounds in mice. There were 32 male mice in 4 different groups. First group was control and the others were experimental. Different dose of watery such as 50, 150 and 300 micro L were injected them. After anaesthetizing the mice, Skin wound is created on the back of the Mice by a 6mm punch. While the Mice in control group were treated by serum physiology, around the wound of the back of other Mice watery of green tea were injected. The researchers measured the dimension and also the recovery percent of the wound in the 1st, 3rd, 7th and 15th days of study. Furthermore they evaluated the duration of recovery. Some histology studies have been done. On 2 Specimen of wound in 3rd/ 7th / 15th days for study. In this way fibroblasts macrophages, Neutrophils and endothelial cell of blood vessels from the wounds were accounted. Data were analyzed using analysis of variance method. Evaluation of recovery processes showed there were no significant differences between groups on 4th day of study. The degree of recovery on 7th day for observer group was [19.2 +/- 10.2] for 2nd group were [38.8 +/- 17.9], for 3rd groups were [48.3 +/- 17.7], for 4th were [60 +/- 17.8] [P < 0.04]. The degree of recovery on 15th day of study for observer group was [25 +/- 8.2] / for 2nd group were [47.8 +/- 17.5] 3rd group [66.5 +/- 18.1] and for 4th group were [76.5 +/- 18.4] [p<0.01]. The degree of fibroblasts in different groups are alternatively as follow in the 1st group [observer] [106 +/- 13.8, 185.8+ 12.1, 90 +/- 10.5], 2nd group [89.1 +/- 20.2, 152 +/- 15.5, 101.6+13.5], 3rd group [97.1 +/- 22.5, 162.9 +/- 16.8. 110.2+10.4] and 4th group [100.2 +/- 21.2, 174.9 +/- 14.5, 121.5 +/- 11.5]. According to the findings, watery of green tea extract speed up the wound healing. We recommend more researches in the field of content enrichment, different methods of use and the effect of them. Examine the water and alcohol of green tea extract on different animals [chronic wounds and burnt] and finally on sick persons

Evaluation of correlation between human papilloma virus and P53 proteins expression in oral squamous cell carcinoma

Head and neck squamous cell carcinoma is the sixth most common cancer worldwide. Inactivation of one or more components of the P53 network is a common event in human neoplasia. In head and neck carcinoma, disability of P53, occurs in a high proportion of cases by mutation in the P53 gene. Cause of inactivation, such as the presence of human papilloma virus [HPV] has also been recognized. The aim of this study was to examine co-incidence of human papilloma viruses type 16.18 and P53 alteration in oral squamous cell carcinoma. Of 42 oral tissue specimen [36 oral squmous cell carcinoma and 6 oral verrucous carcinoma] including 25 men and 17 women which were visited in Isfahan University, School of Dentistry in 1369-1383, multiple serial sections from formalin fixed, paraffin embedded tissues were prepared. Immunohistochemical detection of P53 protein was carried out by a standard Avidin-Biotin peroxidase procedure. Standard PCR analysis was used for detection of DNA HPV 16 and 18. The data were analyzed with Chi-Square and Fischer Exact tests. General HPV sequence was detected in 21 out 42 [50%] tumor specimens. HPV 16 and 18 sequences were detected in 11 of 42 [26.2%] tumor specimens. Over expression of P53 was observed in 23 of 42 [54.8%] oral cancers. Ten of the 21 HPV Positive tumor specimens showed alterations in P53 protein expression. In spite of no significant correlation between observation of general HPV genome and P53 alteration, a significant correlation between HPV 16 and 18 and P53 alteration existed. In present study, we could not show any significant correlation between general HPV genome and intensity of P53 expression. A remarkable correlation between HPV 16 and 18 and over expression of P53 protein was observed. So, the results of this study remained controversial and we suggest a more precise modified procedure to be performed on this subject. So, frozen technique and more samples would be necessary for further study in future