[Treatment effect of onion on sexual behavior after induces an antiepileptic drug [lamotrigine] in male rat]

The medicinal use of onion dates back to ancient China and India. Men with epilepsy have reduced fertility, and antiepileptic drugs may affect semen quality. Disturbances of reproductive endocrine hormones are more often found in men with epilepsy than in the general population. There is an ongoing debate whether this can be attributed to chronic use of antiepileptic drugs or to the epilepsy itself. The aim of the present study was to evaluate the beneficial degree of sexual behavior in male rats after inducement onion in lamotrigine receiving groups. Forty wistar rats, randomized into four groups [n=10], were used for this study, five of them male and five of them female. Animals in Group A served as the control and was drinking distilled water. Animals in Groups B: treated with 3cc/rat of onion juice, in Groups C: received10mg/kg/day lamotrigine, in Groups D: received10mg/kg/day lamotrigine plus 3cc/rat of onion juice. In All treatments were for 5 weeks. 48 hours before end of study estradiol benzoate [25 micro gr/kg/day, s.c.] and 6 hours before end of study progesterone 0.3muM were injected. Results showed the percentage of erections and couplings and serum testosterone in onion treated groups were significantly increased [p<0.01] when compared to other groups. onion and its constituents are stated to has antioxidant. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis in diseases. These findings lead to the conclusion that onion significantly lowered the adverse effects of lamotrigine, and can do beneficial effect on sexual behavior in male rat

Protective effects of Quercetin on spermatogenesis in streptozotocin-induced diabetic rat

Quercetin is a strong antioxidant and long-term treatment of STZdiabetic animals and it has been shown to reduce oxidative stress. antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat [n=40] were allocated into three groups, control group [n=10] and Quercetin [QR] group that received 15mg/kg [IP] QR, [n=10], and Diabetic group that received 55 mg/kg [IP] streptozotocin [STZ] [n=20] which was subdivided to two groups of 10; STZ group and treatment group. Treatment group received 55 mg/kg [IP] STZ plus15mg/kg QR, daily for,4 weeks, respectively; however, the control group just received an equal volume of distilled water daily [IP]. Diabetes was induced by a single intra peritonea injection of streptozotocin [55 mg/kg]. Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for testosterone, TAC, MDA and Ox-LDL levels and testes tissues of Rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis. Sperm population, percentage of sperm viability and motility significantly increased in group that has received 15 mg/kg [IP] Quercetin [P < 0.05] in comparison to control and experimental groups. Since in our study 15 mg/kg [IP] Quercetin have significantly Preventive effect on Sperm percentage of viability and motility by reducing level of Reactive Oxygen Species [ROS] in serum, so it seems that using it can be effective for sperm healthy parameters in Diabetic Rat

Effects of danae racernosa on spermatogenesis in rat

Danae racemosa is used medicinally and as a culinary spice. Its constituents are stated to have antithrombotic, antihepatotoxic, antinociceptive and antioxidant. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat [n=30] were allocated into three groups, control [n=10] and two test groups [each of ten]. Animals in test groups were subdivided into groups of 2 that received fresh extract equivalent to 200 and 400 mg/Kg/Rat/day Danae racemosa extract. Fresh Danae racemosa extract was administered with gavages for 28 consecutive days. Animals were kept in standard condition. On twenty eighth day, the testes of rats in the all groups were removed and sperm was collected from epididymis and was prepared for analysis. The percentage of sperm viability and motility in both test groups significantly increased [p<0.05], but the sperm concentration significantly increased only in the group that received the high dose of Danae. racemosa extracted, [p<0.05]. It was evident that there was no difference on sperm morphology and testis weight in test groups comparing to control group. In our study Danae racemosa extract has significantly affected the sperm number and percentage of viability and motility; it seems that using 400 mg/Kg/Rat/day of Danae racemosa extract is effective in sperm health parameters