[Effect of omega-3 fatty acids on lipid peroxidation and plasma total antioxidant capacity after acute resistance exercise in young male athletes]

Antioxidant supplementation may decrease exercise-induced oxidative stress in athletes. Considering the type of exercise, dose and kind of supplementation the results of the studies in this field are inconsistent. The present study was conducted to assess the effect of omega-3 fatty acids on lipid peroxidation and plasma total antioxidant capacity after acute resistance exercise in athletes. In this randomized double blind clinical trial, twenty healthy subjects were divided into two groups; omega-3 [n=10] and placebo [n=10] groups. Each subject in omega-3 group received 3 capsules of omega-3 [3000 mg] per day and placebo group received the same number of placebo capsules per day for a period of 7 day. All subjects of both groups performed high intensity acute resistance exercise. Blood samples were collected from the antecubital vein 1 week prior to the exercise, immediately pre-exercise and 24 hours post-exercise for determination of the concentration of malondialdehyde [MDA] and plasma total antioxidant capacity. Data were analyzed by repeated measure ANOVA at alpha 0.005]. The results of this study indicated that consumption of omega-3 fatty acid supplements might prevent any increase in the serum level of malondialdehyde after high intensity resistance exercise. Therefore, it may be useful for prevention of oxidative stress-induced damage in young male athletes

Association of C609T and C465T polymorphisms of NQO1 gene with AML

Many studies have demonstrated that polymorphisms of NQO1 including C465T and C609T are associated with increased risk of acute myeloid leukemia [AML]. Our aims are to assess incidence of these polymorphisms in Tehran patients and study the influence of low activity of NQOl in AML. In this case-control study, we used PCR and RFLP analyses to study the prevalence of C609T NQO1 in 140 patients, and C465T NQO1 in 124 patients; there was also a control group of 80 being age-sex matched. We calculated odd ratio with SPSS 16 to examine if these polymorphisms are associated with AML. No significant association between the two common polymorphisms of NQO1 and risk of AML was observed. C609T odd ratio for TT genotype versus CC was obtained to be 0.91 [CI 95% = 0.51-1.63] and for CT versus CC it was 1.06 [CI 95% = 0.57-1.95]. C465T odd ratio for TT genotype versus CC was calculated to be 0.22 [CI 95% = 0.009-5.56] and for CT versus CC it came out to be 3.01 [CI 95% = 0.63-14.32]. Our findings suggest that the NQO1 C609T and C465T gene variants do not have a major influence on the susceptibility to adult AML

[Detection of the YMDD motif mutations rate in chronic hepatitis b patients treated with lamivudine]

Lamivudine is the first nucleoside analogue licensed for the treatment of chronic hepatitis B patients which is effective in suppressing virus replication and results in reduced disease activity. However the most important problem of lamivudine treatment is the emergence of lamivudine resistance strains with amino acid substitution in the YMDD motif of the DNA polymerase during the treatment. The aim of this study was to evaluate the frequency of YMDD motif mutations in chronic hepatitis B patients treated with lamivudine. 56 chronic hepatitis B patients who had not previously received interferon and/or a nucleoside/nucleotide analogue, received lamivudine for a minimum of 6 months were examined in this study. HBV DNA was extracted from serum samples, and YMDD mutations in the HBV DNA polymerase gene was determined using PCR-direct sequencing. HBV DNA quantification was determined using real-time PCR. 56 patients 39 [69.6%] male and 17 [30.4%] female with mean age of 34.12 +/- ] 13.6 [were evaluated. YMDD motif mutations were observed in 15 out of 56 patients [26.7%]. 8 of these mutations [14.2%] were in the form of YIDD and the remaining 7 [12.5%] in YVDD form. Serine mutation at YMDD motif was not found in this study. YMDD motif mutations leading to lamivudine resistance is common in our chronic hepatitis B patients. Molecular diagnosis of YMDD motif mutations using methods such as direct DNA sequencing can detect upcoming viral resistance in chronic hepatitis B patients

Comparision of barry and barry-taguchi ureterovesical reimplantation techniques in kidney transplantations: a randomized clinical trial

Renal transplantation is the treatment of choice for chronic renal failure. Using a suitable uretero- vesical anastomosis technique can prevent most of risks for kidney graft. Extravesical ureteroneocystos- tomy is becoming popular in renal transplantation because of the low complication rate and technical ease. The decreased complication rate is due to limited bladder dissection and the need for a shorter ureteral segment from the donor. In this study we assessed the effectiveness and complications of a new technique, Barry-Taguchi technique and compared it with Barry technique. We recorded all urological complications developed in the recipient's kidney between September 2004 and March 2007 [mean follow-up 12 months] after performing extravesical Barry-Taguchi [new tech- nique] and Barry ureteroneocystostomy. The urological complications studied included complicated hema- turia, urinary fistula, and ureteral stenosis. A total 100 patients who underwent Barry-Taguchi technique and 98 patients who underwent Barry technique were studied. The incidence of urological complications in Barry-Taguchi and Barry re-implan- tation technique was 4% [n=4] and 5% [n=5%], respectively. These complications included 1 urinary leak- age and 3 ureteral obstructions for Barry-Taguchi technique, and 4 obstructions and 1 leakage from Barry group. In both trial groups, no complicated hematuria has occurred. In addition, the recorded time taken for ureteral anastomosis ranged from 4 to 16 [mean 8.3] min for Barry-Taguchi technique and 5 to 20 [mean 9.9] min in Barry technique. The Barry-Taguchi extravesical ureteroneocystostomy technique is a rapid and rather simple tech- nique. Without increasing the incidence of urological complication rate, it is a reliable method for perform- ing ureteroneocystostomy