ABSTRACT
Endometritis is one of the major causes of infertility in mares. The present study was conducted to evaluate intrauterine infusion of Eucalyptus globulus oil as treatment for mares with experimentally induced endometritis. Mares [n=8] were synchronized using two injections of PGF 2alpha [10 mg]14 days apart. All mares were inoculated with a live culture of 5 × 10[6] CFU S. zooepidemicus in the dioestrus phase after the termination of oestrus. The day of inoculation was considered as day 0 of the experiment. On days 3, 6 and 9, cytological and bacteriological specimens were collected to determine endometritis. Mares were randomly assigned to two groups on day 10. Mares in treatment [n=4] and control [n=4] groups received intrauterine 6% Eucalyptus oil solution [100 ml] and intrauterine saline solution [100 ml] for 5 consecutive days. Cytological, bacteriological, and ultrasonographic evaluations were implemented at days 15, 17 and 19 [1, 3 and 5 days after termination of intrauterine treatment, respectively]. The number of mares diagnosed with endometritis, the depth of their intraluminal uterine fluid and the number of infected mares did not change by the treatment [P>0.05]. In conclusion, the 5-day intrauterine infusion of 6% E. globulus oil solution failed to treat experimental endometritis in mares
ABSTRACT
Immune-mediated hemolytic anemia [IMHA] is characterized by the destruction of erythrocytes or sometimes bone marrow erythroid precursors mediated by immunoglobulins [IgG, IgM], with or without complement [C[3]]. The main objectives of this study were to assess the laboratory test results of IMHA and to investigate its possible underlying causes in cats referring to the Veterinary Teaching Hospital of Tehran. The Coombs' test [CT] was performed in 74 cats with PCV below 0.35. The test was positive in 26 cats. These positive CT cats were categorized into four groups based on the PCV ranges and type of anemia including: Group A: 9 of 26 cats had nonregenerative anemia [PCV median, 0.22] [1 feline leukemia virus positive which had erythroleukemia, 1 feline infectious peritonitis positive, 1 with Hemoplasma spp., 3 with renal failure, 2 with inflammatory disease and 1 with no diagnosis]. Group B: 4 cats [PCV median, 0.31] had a regenerative anemia with severe dehydration. Group C: the other 9 cats with a normal range of PCV [median, 0.34] involved with various conditions [vaccination, parturition, acetaminophen poisoning, osteoporosis, and renal failure]. Group D: the remaining 4 cats with a marginal range of PCV [median, 0.30] had a history of inflammatory disease and drug therapy. The results of this study indicated that infectious diseases and drug therapy were the main factors associated with positive Coombs' test results
ABSTRACT
From the winter 2002 to spring 2006, 126 moribund rainbow trout with clinical signs of external body haemorrhages around and within the oral cavity were sampled from 10 rainbow trout farms situated in the northwest and west of Fars province, Iran and examined for the detection and identification of Yersinia ruckeri, the causative agent of enteric redmouth disease. Fish kidneys were cultured aseptically on brain heart infusion [BHI] agar plates and incubated at 25°C for 48 h. Using conventional biochemical tests, Y. ruckeri was detected in 7 fish [5.5% of total fish sampled]. This was also confirmed using specific polymerase chain reaction [PCR] assay. The 16S rDNA PCR assays produced amplicons of 409 bp when applied to Y. ruckeri isolates as well as a reference strain. Results of antibiogram tests on Y. ruckeri isolates showed a high susceptibility to enrofloxacin, norfloxacin, ofloxacin, trimethoprim and oxytetracycline. In pathogenicity tests, dilution of 4 x 10[8] colony forming unit/ml of Y. ruckeri by immersion route in challenge experiments showed 70 +/- 8.2% mortality during 14 days post-infection. Experimentally infected fish showed typical haemorrhages in mouth, blackening of skin, exophthalmia and a wide haemorrhages on the internal organs