Comparison of salivary anti helicobacter pylori IgG serum IgG with and bacteriological tests in detecting helicobacter pylori infections

This study was conducted to compare the efficacy of enzyme-linked immunosorbent assay [ELISA] for detecting anti-Helicobacter pylori [H. pylori] specific IgG antibodies in specimens of oral fluid and serum with bacteriological tests. Antral biopsy specimens, as well as serum and oral fluid samples were collected from 97 patients who underwent upper gastrointestinal endoscopy. The presence or absence of current H. pylori infection was determined by culture, histology and urease detection. Anti-H. pylori specific IgG was detected in serum and oral fluid, using an established lab-made, and a commercial ELISA kit. The obtained data were compared with results of bacteriological tests. In all, 62 [64%] of 97 patients were positive for H. pylori by one or more of the gold standard tests [culture, histology and urease detection]. Lab-made enzyme-linked immunoassay of oral fluid had a sensitivity and specificity of 92% and 83% respectively. A sensitivity and specificity of 87% and 83%, respectively, was obtained with the commercial kit. Lab-made enzyme-linked immunoassay of serum samples had a sensitivity and specificity of 90% and 88%, respectively. A sensitivity of 86% and specificity of 86% was obtained with the commercial kit. Detection of anti-H. pylori specific IgG in oral fluid by ELISA is comparable in sensitivity and specificity with serum based methods. Oral fluid based ELISA could provide a reliable, non-invasive method for the diagnosis of H. pylori infection. Saliva testing may have a role in epidemiological studies

[Determination of Brucellamelitensis biotypes in human and sheep isolates]

Brucellosis is one of the most important zoonsis. Because of the importance of this disease and the effect of strain variation on strategic planning for the control of this disease, sheep and human Brucella isolates were evaluated for strain variation. Five human blood Brucella isolates and 25 sheep embryo Brucella isolates were collected from hospitals and veterinary centers in Isfahan. Total genomic DNA was extracted from the collected samples using method of SDS and proteinase K treatment, phenol/chloroform extraction and ethanol precipitation. omp2a and omp2b fragments of all isolates were amplified using 2 pairs of specific primers [omp2a R, F and omp2b R, F] and the PCR products were electrophoresed and stained. These PCR products were then restricted using Alu I, Hinf I and TaqI restriction endonuclease. The PCR products of all human and sheep isolates had the same size 1100bp for omp2a and 1200bp for omp2b. The banding pattern of PCR-RFLP for all of the isolates was similar to banding pattern of the Brucellamelitensis biotype 1. Based on the banding pattern of PCR-RFLP of the omp2a and omp2b fragments, it can be concluded that all the studied samples in Isfahan are Brucellamelitensis biotype 1. This study should be repeated regularly. This will inform us if new species or biotype of Brucella have entered to the region. This is important in planning for the control of the disease