Global genome methylation status in gastritis lesion in comparison with normal adjacent tissue and its relationship with clinicopathologic findings

To determine the role of global genome methylation in gastritis lesion and its relation with clinicopathologic finding. The study was conducted on 44 gastritis and normal adjacent specimens using a technique composed of restriction enzyme digestion and pyrosequencing known as LUMA [LUminometric Methylation Assay]. At first, DNA extracted from gastritis lesion and normal tissue was digested with HpaII [sensitive to methylation in recognition site] and MspI [insensitive]. These enzymes leave an overhang after cutting which are then filled in a polymerase extension assay with stepwise addition of dNTPs using pyrosequencing. The comparison of the height of picks obtained form both enzymes provides the possibility to evaluate and compare global genome methylation level of normal and gastritis tissues. If the target site is fully methylated, the HpaII/MspI will approach toward zero .If not, this ratio will go around one. In the other conditions the ratio varies between 0-1. According to our findings, gastritis tissue was significantly more hypomethylated [p = 0.04] than the nornal tissue and Global genome methylation had no correlation with sex, age, microsatellite instability [MSI] and gastritis severity. Global DNA hypomethylation occurs in the gastritis lesion. Presumably the process of hypomethylation keeps falling in the next steps leading to gastric cancer

Endoscopic treatment of a bronchobiliary fistula due to complicated hydatid cyst after surgical intervention: a case report

Hydatid cyst is an infectious parasitic disease often caused by Echinococcus granulosus, or to a lesser extent by Echinococcus alveolaris. The liver is the organ most frequently involved. We report a case with hydatid cyst of the liver and lung that many years after surgery developed bronchobiliary fistula and biloptysis. The patient had been operated 2 times previously but yet the problem was present. Endoscopic retrograde cholangiopancreatography [ERCP] was used successfully for treatment. We present the result of ERCP and endoscopic sphincterotomy in the management of biliary hydatid disease. Bronchopleural fistula after hydatid cyst surgery could be cured by ERCP and sphincterotomy with or without stent insertion

Relation between Helicobacter pylori and nausea and vomiting in pregnant women

Gestational nausea and vomiting in pregnant women have a high prevalence. The disorder will usually be better after the third trimester but, in some cases, it persists throughout pregnancy and makes the life of pregnant women disturbed. This case-control retrospective study was conducted to determine the relation between Helicobacter pylori and nausea as well as vomiting in pregnant women referring to the healthcare centers affiliated to Mashhad University of Medica Sciences in 2006. 187 pregnant women at the third trimester of their pregnancy were selected. According to the experience of nausea and vomiting from the first trimester, they were divided into two groups: 89 women with the disorder and 98 women without it. A questionnaire in three parts was used for data collection: demographic information, history of nausea and vomiting, and socioeconomic status. The questionnaire was validated by context validity and reliability by test-retes method. Anti-Hp immunolobin G [IgG] serum antibody wastested by ELISA it determine seropositivity [r=0/89]. The result showed a significant relation between helicobacter pylori and nausea and vomiting during pregnancy. The subjects did not have significant differences in terms of height, job, education of spouse, husbands' job, pregnancy and BMI except for age and socioeconomic status [P=0.037 and P<0.001 respectively]. The results may show that nausea and vomiting during pregnancy can be prevented by diagnosing helicobacter pylori infection in women. Also, it seems that there should be a training program in knowing Helicobacter pylori infection and its complications. Considering the results of this research, studying women with nausea and vomiting during pregnancy for helicobacter pylori infection is advisable

[Association of the vitamin D receptor gene polymorphisms with inflammatory bowel disease in Iran]

Different genes such as vitamin D receptor [VDR] gene have some roles in IBD susceptibility. Some studies have recognized the relation of VDR gene polymorphisms with inflammatory and autoimmune disorders. Determining the frequency of these polymorphisms and their possible relation with IBD can improve understandings about genetic background of these diseases. The objective of this study was to assess the association of VDR gene polymorphisms [Apa I, Taq I, Bsm I, Fok I] with IBD in Iran. In this case-control designed study 100 UC, 50 CD patients and 150 sex and age matched healthy controls, hospital base, were selected. These patients were referred to [Taleghani Hospital] during a one year period [2004-2005]. Assessment of VDR gene polymorphisms was performed by PCR-RFLP method. Only the frequency of the Fok I polymorphism was significantly higher in UC and CD groups. The frequency of the polymorphic allele f was higher in UC and CD groups comparing with controls [p=0.019, OR=1.581 and p<0.001, OR=2.642, respectively]. The f/f genotype was significantly more frequent in UC and CD patients comparing with controls [p=0.010, OR=2.774 and p<0.001, OR = 5.947, respectively]. There were no significant differences between frequencies in patients and controls in other polymorphisms. There is a relation between Fok I polymorphism in VDR receptor gene and IBD in Iran but no association was observed with other 3 polymorphisms

Calculation of residual volume by spirometric data

The current practice to measure RV is either by BPG or helium dilution methods which may not be available in all clinics due to their cost. This paper outlines a method for both direct and indirect calculation of RV via PFT with acceptable sensitivity [81%, 60%], specificity [71%, 94%] and validity [76%, 78%] for obstructive and restrictive lung disease respectively at a much lower cost