Chromosomal studies on laboratory rats [Rattus Norvegicus] exposed to an organic solvent [cyclohexanone]

Cyclohexanone, an aromatic hydrocarbon, was tested for its ability to induce chromosomal abnormalities in male laboratory rats, [Rattus norvegicus], 6, 24 and 48 hours after subcutaneous injection of three doses: 0.1, 0.5 and 1.0 g/kg. The effect of these treatments was scored cytologically in metaphase plates of bone marrow cells. The results showed that the compound was effective in the induction of chromosomal aberrations at all doses and time intervals. The induced abnormalities increased with dose and decreased with time. Qualitatively, they consisted of chromatid gaps and breaks, centric fusions, centrometric attenuation, chromatid exchanges and polypioidy. It was concluded that cyclohexanone could exert harmful effects on mammalian chromosomes, and might accordingly be genetically hazardous to man

Effect of novalgin on chromosomes of rattus norvegicus

Novalgin; the sodium sulphonate derivative of aminopyrine, is an analgesic, antipyretic drug, widely used in Egypt. In spite of its well known hematologic complications, mutagenicity tests are deficient in the literature. The main aim of the present work was to study the cytogenetic effects of novalgin on bone marrow cells of Rattus norvegicus, using the method recommended by the Ad Hoc Committee of Mutagenicity Testing [1972]; Novalgin induced significant Chromosomal aberrations of both chromatid and chromosome type, and depression of mitosis 48 hours after treatment with the maximum tolerated dose in the acute study and after sub-acute treatment with the clinical dose, The results of the study suggest that novalgin is mutagenic and causes depression of mitosis when administered at very high doses, or after prolonged use of the clinical dose

Biochemical and chromosomal studies on rats injected with captan

The influence of acute treatment with captan on nucleic acid and protein content in brain and liver, as well as on chromosomal abnormalities and mitotic division in bone marrow was studied in laboratory rats. Captan was injected intraperitoneally at two dosage levels [5 and 50 mg/ 100 g body wt.], rats were sacrificed 6, 24 and 48 hours after treatment. Administration of captan at dose I [5 mg/100 g] has no significant effect on brain nucleic acid and protein. In liver a significant reduction was observed in DNA at 24 and 48 hours, and in RNA at 48 hours after injection. Dose II [50 mg/100 g] produced a significant decrease in DNA and RNA in both organs after 6 and 48 hours, while their level was elevated after 24 hours in both organs. At the same dose, protein content was considerably decreased in brain and liver after 24 and 48 hours of treatment. Mitosis was significantly increased after 6 hours of captan [50 mg/100 g] injection, while at 24 and 48 hours the increase did not reach the significant value. Chromosomal abnormalities were observed at all periods of the study, the higher dose of captan causing more aberrations than the lower one. End to end association was the most frequent aberration, followed by centric fusion. Gaps were mainly observed in the treatment with the higher dose. Breaks never reached statistical significance. It can be concluded that captan has a marked effect on nucleic acid and protein content, cell division and chromosomal abnormalities