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1.
Egyptian Journal of Veterinary Science. 2008; 42: 1-10
in English | IMEMR | ID: emr-97507

ABSTRACT

The prevalence of anti-Toxoplasma gondii antibodies was investigated in goats slaughtered for food in various areas of the Giza province. Egypt. Two hundred and thirty blood samples collected from the slaughtered goats were used for the serologic diagnosis of T. goadii infection by the modified agglutination test [MAT] with cut-off value 1:25. The antibodies to T. gondii good/i were found in 44.3% [102/230] of goat sera. Experimental infection of goats with the infective stages of both locally isolated and RH strains of T. gondii was carried out. Persistence of T. gondii tissue cysts and lesions were demonstrated in the different tissues and organs of the experimentally infected goats by the histo-pathological examination, This study is the 1st assay of toxoplasmosis in Egyptian goats by MAT and is the unique successful determination of the T. gondii infective stages within the experimentally infected goat tissues by the histo-pathological examination. Consequently the finding results obtained scope the public health significance of goat meat as source of human infection


Subject(s)
Animals , Goats/blood , Agglutination Tests , Animal Experimentation , Heart/pathology , Tongue/pathology , Diaphragm/pathology , Muscles/pathology , Histology
2.
Egyptian Journal of Veterinary Science. 2008; 42: 31-38
in English | IMEMR | ID: emr-97510

ABSTRACT

Toxoplasma gondii, is a ubiquitous apicomplexan parasite of warm-blooded animals. intracellular protozoan and is one of the most common parasitic infections of human, hi the present study diagnosis of Toxoplasma gondii was directed towards the serological methods including enzyme-linked immunosorbent assay [ELISA], and indirect hemagglutination assay [IHat]. The comparative serological examination of 320 sheep and 306 goats blood serum antibody body titres against Toxoplasma gondii were analyzed at 10 settlements in the Giza region, Egypt. The IHat showed the higher prevalence of toxoplasmosis 47.5% in sheep and 59.5%in goats respectively with antibody titres ranging front 1:20 and 1:640'. Also ELISA seropositive samples 44% in sheep and 55.5% in goat respectively. It could be concluded that the IHAT and ELISA tests are an efficient diagnostic tools for detection and selective diagnosis of Toxoplasmosis in sheep and goats using local strain isolate in Egypt


Subject(s)
Animals , Goats/blood , Sheep/blood , Serologic Tests/methods , Enzyme-Linked Immunosorbent Assay , Hemagglutination Tests , Epidemiology , Comparative Study
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