[Effect of ethanolic and watery extract of aerial parts of Stachys lavandulifolia on Trichomonas vaginalis, in vitro]

Trichomonas vaginalis is the most common curable sexually transmitted disease world wide. In view of increased resistance of the parasite to classical drugs of the metronidazole family, the need for new unrelated agents is increasing. In Charmahal va bakhtiare province Stachys lavandulifolia traditionally used for vaginal infection. The study evaluates anti trichmonas activity of of ethanolic and watery extract of aerial parts Stachys lavandulifolia. This study has been carried out as double blind in test and control groups. Etanolic and watery were extracted by hydro distillations. The parasite was isolated from vagina. Sample were collected from vaginal discharges of six patients and cultured in laboratory. Identification were done through direct smear preparation, parasite was added to the 9 test tubes containing metronidazole, etanolic and watery extraction of Stachys lavandulifolia with concentration of [10, 50, 100, 200, 500 and 1000microL/ml] order to determine the effect of these concentration within 72 hours. Finding suggested that the Trichomonas vaginalis could be alive in TYIS-33, watery and ethanolic extraction within 72 hours. In presence of metronidazole for no alive parasite was detected after 72 hours. Number of parasite in TYIS-33, watery [10, 50, 100, 200, 500 and 1000microg/ml] and ethanolic extraction [10, 50, 100, 200, 500 and 1000microg/ml] and ethanol was 1504, 1504, 100, 1304, < 100, 577 respectively. Finding demonstrates further studies are required of of Stachys lavandulifolia to evaluate its microbicidal activity against other sexually transmitted infection

[Effects of eucalyptus camaldulensis exracts on trichimonas vaginalis growth in vitro]

Trichimonas vaginalis is a flagellate protozoa that cause trichomoniasis. Metronidazole which is drug of choice for treatment of this infection has many side effects so the patients are unsatisfied with its using, so it is necessary to seek for alternative drugs. Eucalyptus camaldulensis herb with strong anti-microbial affects is considered as an alternative drug. Investigating the effect of Eucalyptus camaldulensis as an alternative drug Trichomonas vaginalis in vitro. Eucalyptus camaldulensis extraction was prepared using perculasion method. In test tubes containing parasite culture medium different concentrations of the plant extract were added. In other test tubes metronidazole or extract solvent as controls were added. 100 live Trichomonas vaginalis added to each tube and all tubes kept in 37°C and tested for presence of the parasite every 24 hours up to 72 hours. For this purpose 10 microliter of each tube examined under microscope. in tubes with higher concentration of Eucalyptus camaldulensis extract and also in tube containing metronidazole no parasite was observed. In negative control tubes parasite had a normal growth. According to results of this study Eucalyptus camaldulensis extract had strong effects on Trichomonas vaginalis growth. Further investigation is recommended to use this plant as alternative drug for treatment of trichomoniasis