ABSTRACT
The present work was planned to develop a modified system for the growth of C. tetani and production of tetanus toxin, the system includes growth media that contain no meat or dairy by-products using non-animal based products. Hydrolyzed soy proteins have been found to replace the meat/dairy product for growth of C. tetani and the production of its toxin, these replacements resulted in production of high tetanus toxin titer and of low side effects
Subject(s)
Animals, Laboratory , Soybean Proteins , Mice , Lethal Dose 50ABSTRACT
To assess the incidence and prognostic significance of MYB gene [6q23] deletion among a sample of Egyptian B-chronic lymphocytic leukemia [B-CLL] patients and to con elate it with other established prognostic factors This study was conducted on 63 newly diagnosed Egyptian B-CLL patients attending Am Shams University Hospitals. Fluorescence in situ hybridization [FISH] using locus specific identifier MYB [6q23] probe was applied on peripheral blood/bone marrow samples and was correlated with different established prognostic factors. Follow up was done through a period of 48 months to evaluate disease outcome. 6 patients had MYB deletion. All of them showed lymphocyte doubling time [LDT] more than 12 months and were CD38 positive. 83 3% of patients were good responders to therapy and none of these I atents died. B-CLL patients who were positive for MYB [6q23] deletion represent a cytogenetic entity associated with CD38 positivity and LDT>12 months They were considered as an intermediate risk group