ABSTRACT
Background: hydatidosis is one of the zoonotic diseases which affect animals and human beings at the larval stage of Echinococcusgranulosus, thereby playing a role in exacerbating the economic and health problems in Iran
Objectives: this study was conducted to investigate the strains of Echinococcusgranulosus isolated from cattle hydatid cysts, in Khorramabad, in the Lorestan Province, in the west of Iran
Methods: twenty-six isolates of hydatid cyst of cattle from lung [24 samples] and liver [2 sample] organs were collected from Golshan Slaughterhouse, in Khorramabad. All of the samples were transferred to the laboratory for morphometric characterization and molecular study. In morphological characterization, blade length of large [LBL] and small [SBL] hooks and the ratio of blade length to total length in large [LBL/LTL] and small [SBL/STL] hooks and total length of large [LTL] and small [STL] hooks were measured. In molecular study, a partial sequence of cytochrome oxidase 1 [CO1] with 440 bp in length was amplified applying primers J3 and J4.5. Genomic DNA sequencing was performed by Sanger's method
Results: the morphological results showed that there is no significant difference between isolated from cattle hydatid cyst and sensu stricto strain [p<0.05]. The results of molecular studies support the findings of morphological characterization. All sequences showed 100% identity with sensu stricto strain
Conclusions: the results from this study showed that sensu stricto strain [G1] is a causative agent of cattle hydatid cyst in Khorramabad. On the other hand, the cattle play a role in enabling the parasite to complete its cycle. Hence in order to execute a control program for minimizing the effects of this disease, the cattle should be considered as a source of infection for dogs. The results of this study could be helpful in designing such control program in the region
ABSTRACT
In order to deworm the ruminants especially of sheep in Iran, consumption of benzimidazoles has more than 2 decades history and today farmers are using imidazothiazoles, macrocyclic lactones and mostly benzimidazole compounds [BZs] to treat infected farm animals. It has been demonstrated that the most common molecular mechanism leading to BZ sresistance in Haemonchus contortus is a single mutation at amino acid 200 [phenylalanine to tyrosine] of the isotype 1 of beta tubulin gene. According to the report of such mutations in Iranian Teladorsagia circumcincta isolates with Restriction Site Created PCR-RFLP, we decided to evaluate the frequency of such mutations in H. contortus in three different geographical areas of Iran. A total of 102 collected adult male H. contortus were evaluated with PCR-RFLP [using PSP1406I as restriction enzyme]. By means of a second step to compare function of different methods and to increase sensitivity of detection mechanism, a third of samples were examined by another PCR-RFLP method [using TaaI as restriction enzyme] and finally beta tubulin gene of two samples was sequenced. All of samples were detected as BZss homozygote. Finally, beta tubulin gene sequencing of two samples showed no point mutation at codon 200. It seems that BZ resistance of H. contortus in Iran is not a serious problem as anticipated before