ABSTRACT
Background: ATP-binding cassette transporter A1 [ABCA1] is a membrane integral protein which plays a vital role in High Density Lipoprotein [HDL] metabolism and exerts a protective effect against Hypoalphalipoproteinemia [HA] by mediation of rate-limiting step in HDL biogenesis. In addition, this protein possesses anti-inflammatory effects by inhibiting the production of some inflammatory cytokines in macrophages. This study investigated the association of ABCA1-565 C/T gene polymorphism with HA and serum lipids, IL-6 and CRP levels
Methods: A population which consisted of 101 HA and 95 normal subjects were genotyped for ABCA1-565C/T polymorphism by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism [PCR-RFLP]. The serum concentrations of lipids, IL-6 and high sensitive-CRP [hs-CRP] were measured by the relevant methods
Results: The frequency of T allele was significantly higher in the HA group than the controls [31.7 vs. 19.5%, p=0.002]. Thus, carriers of the T allele [CT and TT genotypes] had a higher risk for HA [p=0.016, OR=2.04, 95% CI=1.14-3.63]. T allele carriers demonstrated decreased HDL-C and increased triglyceride, IL-6 and CRP levels than those with the CC genotype
Conclusion: This study suggests that the-565 C/T polymorphism of ABCA1 gene is associated with an increased risk of HA, decreased HDL-C and increased TG, IL-6 and CRP
Subject(s)
Adult , Female , Humans , Male , Middle Aged , ATP Binding Cassette Transporter 1/genetics , Polymorphism, Genetic , Genetic Association Studies , Interleukin-6/blood , C-Reactive Protein , IranABSTRACT
Polycystic ovary syndrome [PCOS] is a common endocrine / metabolic disorder in women of reproductive age .Abdominal adiposity and obesity are frequently present in PCOS. It now appears that, obesity is associated with a low-grade inflammation of white adipose tissue. Adipokines play a significant role in the pathogenesis of a low-grade inflammation associated with obesity. Among variety of adipokines, resistin and visfatin are proposed as important pro inflammatory mediators and they have recently been suggested to be associated with obesity related diseases. The aim of this study is to evaluate the correlation of visfatin and resistin serum levels and the ratio of these two adipokines with BMI in PCOS women under age of 35 years old. Twenty eight young women with clinically confirmed PCOS disease [14 lean and 14 obese], and 12 young, healthy and lean women with stable weight and BMI<25 were enrolled. Blood was obtained from the included persons, and visfatin and resistin were assessed by ELISA method. We did not observe any significant differences in serum visfatin and resistin concentrations and also in the Visfatin/Resistin ratio between PCOS and control group. Also we did not found a significant correlation between visfatin and resistin with BMI. This study demonstrated that serum resistin and visfatin levels do not seem to be directly involved in the pathology of PCOS
ABSTRACT
Teucrium polium can reduce serum glucose. There are few reports in the literature related to this subject and the resolution of this mechanism requires further experiments. The aim of the present study was to evaluate the effects of Teucrium polium aerial parts extracts on oral glucose tolerance tests and pancreas histology in streptozocin-induced diabetic rats. In order to prepare the aqueous concentrate, aerial parts extract was dissolved in distilled water and was boiled for 30 minutes. For the preparation of ethanolic solution, powder was dissolved in ethanol and mixed by a shaker. Diabetic rats were induced with single IP injection of streptozotocin [STZ] at a dose of 50 mg/kg body weight dissolved in normal saline just before use to the 16 hr fast rats. Both groups, diabetic and normal were sacrificed by ether anesthesia. The tissue samples were formalin fixed and paraffin embedded for microscopic examination in accordance with routine laboratory procedures. Blood was collected from the tail vein of the rats. Serum glucose levels were then measured by commercial kits by using a glucose oxidized method. There were no biochemical abnormalities or histological changes in the pancreas of control rats. Post treatment of Teucrium polium aerial parts extract reduced the severity of streptozotocin diabetic pancreases. Our histopathological investigation along with the biochemical evaluations showed a significant effect on histological changes in the pancreas of induced diabetic rats upon Teucrium polium aerial parts extract treatment [P<0.05].
ABSTRACT
To determine whether granulocyte colony-stimulating factor [G-CSF] could reverse liver damage in the model of acute liver injury induced by carbon tetrachloride [CCL4]. Prospective, using experimental animal model of acute and chronic liver injury. Babol University of Medical Sciences, Babol, Iran. We established an animal model of liver damage by administration of CCL4 [1 ml/kg, IP]. Two hours later the animals were treated with G-CSF [100 microg /kg body weight, IP]. On the 28[th] day, rats were scarified. Malondialdehyde [MDA] was determined using diagnostic kits following recommendations of manufacturer of the kits. Serial 5 micro m thick liver sections were stained with haematoxylin-eosin and Masson,s trichrome and examined. Reduction in serum albumin and total protein levels [1.24 +/- 0.16 and 3.22 +/- 0.21 g/ dl, respectively] were 2.58 +/- 0.19 and 6.82 +/- 0.30 g/dl, respectively reversed by G-CSF treatment. CCL4-induced increase in serum AST, ALT, and ALP activities and MDA and hydroxyproline levels were significantly suppressed by G-CSF treatment. G-CSF stimulates liver repair and may be clinically beneficial in restoring liver damage. There was a positive correlation [p < 0.05]between histopathological and biochemical parameters