ABSTRACT
This study presented as attempted to produce a biological control agent Beauveria bassiana [Balsoma] Vuillemin fungus against insects pests such as the green peach aphid Myzus persicae [Sulzer] the basic elements necessary for production of the bio-cidal agent were determined according to the recominded methods. The results showed that the best fermentation medium for growth of fungus B. bassiana, was consisted of two - extracts of potato and rice seeds, the concentration of fungal spores in volume unit [ml] was 27.0 x 10 8 spore/ml, while it was reduced to 22.0 x 10 8 spore/ml, and 21.8 x 10 7 spore/ml, in both media, potato extract and rice extract respectively. The efficiency of some substances such as [Kaoline, Rice seeds, Calcium carbonate and Talic] were tested as carrier substances to fungi. It was found that the Kaoline was appeared a better effective than the rice seeds with regard to the fungal concentration in weight unit [gm], reached to 17.0 x 10 7 spore/gm, while it was reduced to 10.0 x 10 6 spore/gm, in rice seeds substance, while two-substances Calcium carbonate and Talic were failed as substances carrying fungal inoculum. The percentage of [100] ml inoculum/[100] gm Kaoline was showed high significant results of the concentrations of fungal in weight unit [gm], reached to 17.5 x 10 7 spore/gm. The results of the laboratory and field - work evaluation referred to efficiency of the bio - cidal as follow:- 1- There is no change on the concentration of the fungal spores in weight unit [gm] of bio - cidal during the assigned storing period which lasted for six - months of storing reached to 17.0 x 10 7 spore/gm, in the first step of production and its continue to the end of storage period. 2. Whole concentrations of bio - cidal was appeared high efficiency on the average of mortalities of green peach aphid, Myzus persicae. 3- The bio - cidal was proved to have a high ability to use as a biological control against green peach aphid, in field experiment which was resulted in control against green peach aphid, in field experiment which was resulted in [80.31 percent] a mortality, when was compared with control treatment [0 percent]