ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects of iNOS gene transfer on portal hypertensive rats.</p><p><b>METHODS</b>Eukaryotic expression plasmid pcDNA(3)/iNOS was used to transfect sinusoidal endothelial cells (SEC) mediated by Lipofectamine. Transfection rate and gene exspression were detected. Hepatic cirrhosis was induced in male Sprague-Dawley rats by intraperitoneal injection of carbon tetrachloride, and the cirrhotic rats were divided into three groups:Liposome-pcDNA(3)/iNOS (n = 10), Tris buffer (n = 10) and nude plasmid (n = 10), which were injected into the portal vein of experiment cirrhotic rats respectively. Five days later, animals were killed, immunohistochemistry and spectrophotometry methods were used to measure the expression of iNOS and the amount of NO production.</p><p><b>RESULTS</b>Eukaryotic expression plasmid pcDNA(3)/iNOS could effectively transfect SEC and express corresponding gene products. Following iNOS gene transfer, compared with the two controlled groups, iNOS expression and the NO production were significantly increased meanwhile portal pressure was decreased significantly.</p><p><b>CONCLUSIONS</b>The iNOS gene transfer is a feasible and an effective approach to treat portal hypertension in cirrhotic rats which could increase the expression of intra-hepatic iNOS and the amount of NO production thus leading to a remarkable reduction of portal venous pressure.</p>
Subject(s)
Animals , Male , Rats , Carbon Tetrachloride , Toxicity , Disease Models, Animal , Hypertension, Portal , Therapeutics , Immunohistochemistry , Liver , Chemistry , Pathology , Liver Cirrhosis , Nitric Oxide Synthase , Genetics , Metabolism , Nitric Oxide Synthase Type II , Portal Pressure , Rats, Sprague-Dawley , Spectrophotometry , Transfection , Methods , Treatment OutcomeABSTRACT
Succinic acid is widely used in the field of chemistry and food proces s. Compared to the traditional chemical synthesis, succinic acid production by fermentation has some benefits. The new process with competitive cost uses rene wable agricultural resources including green house gas CO 2 instead of fossil f uels and reduces pollution from chemical synthesis. In this article, research progr e ss in production of succinic acid by fermentation such as microorganisms, metabo lic pathways and its regulation, fermentation and isolation are briefly reviewed .