High resolution melting analysis for detecting of JAK2V617F mutation in patients with myeloproliferative neoplasms / 中国实验血液学杂志

This study was purposed to investigate the feasibility of high resolution melting (HRM) in the detection of JAK2V617F mutation in patients with myeloproliferative neoplasm (MPN). The 29 marrow samples randomly selected from patients with clinically diagnosed MPN from January 2008 to January 2011 were detected by HRM method. The results of HRM analysis were compared with that detected by allele specific polymerase chain reaction (AS-PCR) and DNA direct sequencing. The results showed that the JAK2V617F mutations were detected in 11 (37.9%, 11/29) cases by HRM, and its comparability with the direct sequencing result was 100%. While the consistency of AS-PCR with the direct sequencing was moderate (Kappa = 0.179, P = 0.316). It is concluded that the HRM analysis may be an optimal method for clinical screening of JAK2V617F mutation due to its simplicity and promptness with a high specificity.

correlation between gatifloxacin's acute adverse reaction and intravenous drip velocity

To investigate the correlation between acute adverse reaction [AAR] and intravenous drip velocity of gatifloxacin. Patients who had received intravenous gatifloxacin [0.2 g/100ml] infusion in the Emergency Center, Zhongnan Hospital, Wuhan University, Wuhan, China from January to August 2012 were enrolled in this study. Patients were randomly divided into 3 groups according to different drip velocities: Group A: velocity

Using three-dimensional CT to guide Le Fort I osteotomy in maxillary retrognathism patients / 中华整形外科杂志

<p><b>OBJECTIVE</b>To analysis the maxillary bony structures by three-dimensional CT in maxillary retrognathism patients so as to provide information for Le Fort I osteotomy.</p><p><b>METHODS</b>20 maxillary retrognathism patients underwent Le Fort I osteotomy, while 20 patients with simple mandibular fractures were included as control group. All the patients received Skull 3-D CT before operation. The measurement about descending palatine artery and wing palatal was performed by Surgicase 5.0. The data were analyzed statistically.</p><p><b>RESULTS</b>The average distance from the piriform aperture margin to wing palatal tube was (33.74 +/- 6.74) mm in the retrognathism group; while (35.67 +/- 7.50) mm in the control group, showing a significant difference between the two groups (P < 0.05), but there was no statistically difference in the height of pterygomaxillary junction between the two groups.</p><p><b>CONCLUSION</b>The safe depth for Le Fort I osteotomy in patients with hypoplasia maxilla is 32 mm. CT scanning can provide guidance for osteotomy.</p>

Treatment of fracture of tibia and fibula with three dimensional diaplasis fixation / 中国骨伤

<p><b>OBJECTIVE</b>To study the clinical effect of three dimensional diaplasis fixation in fracture of tibia and fibula.</p><p><b>METHODS</b>Twenty-one cases of fracture of tibia and fibula were treated with three dimensions fixation (12 males, 9 females, with an average age of 46 years). There were 5 cases in open fracture, 16 cases in closed fracture, and 4 cases in up-segment fracture, 8 cases in mid-segment fracture, 9 cases in below-segment fracture. Oblique fracture were in 10 cases, thrypsis were in 8 cases, multisegmental fracture were in 3 cases.</p><p><b>RESULTS</b>(1) Conditions of diaplasis fracture: dissected diaplasis were in 11 cases, closely dissected diaplasis in 9 cases, functional diaplasis in 1 case. (2) Clinical healing time: the minimum time was 43 days and maximum time was 85 days with an average of 62 days. (3) Conditions of functional recovery: all the patients were followed up from 4 to 12 months, 13 cases were excellent, 8 cases were good. (4) Time of backouting three dimensional diaplasis fixation: the minimum time was 6 weeks and the maximum 12 weeks with an average time of 8.5 weeks.</p><p><b>CONCLUSION</b>The three dimensional diaplasis fixation and the fracture extremity from such a three dimensional solid that it can satisfy crus biomechanics for treating fracture of tibia and fibula with unstressed barrier and uncentric stress. Moreover, the three dimensional diaplasis fixation is elastic, it's structure is so fixed that it can be favorable for bone union.</p>

Electrophysiological findings for 106 cases with auditory neuropathy / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To analyses the clinical characteristics and electrophysiological finding of 106 patients with auditory neuropathy (AN). Investigate the differential curve type of pure tone audiogram and the abnormal ABR.</p><p><b>METHODS</b>Review the history of patients, pure tone audiometry, middle ear acoustic reflexes, auditory brainstem response, distortion product otoacoustic emission and radiologic imaging studies of the brain of 106 patients with AN during December 2001 to May 2007 in retrospect.</p><p><b>RESULTS</b>The 106 patients with AN were of both genders. The age were between 11-37 years old, and the average age was 17.5 years old. The most patients were adolescence (70.8%). Twelve cases of the 106 patients had evidence of other peripheral neuropathy in addition to hearing loss. Another 94 patients were idiopathic origins. The pure tone audiogram showed a minimal to moderate sensorineural hearing loss at low frequencies 0.5 kHz and 0.25 kHz in 209 ears (98.6%). The average hearing threshold (WHO 1997) in 23.2% of disordered ears at less than 25 dB in the "normal" range. Auditory brainstem response could not be recorded in 124 ears (58.5%) at maximum stimulus. The other 88 ears showed 1 or 2 wave, but the wave were small. There were 23 patients which one side ear ABR was 1 or 2 small waves presented, but the contralateral side were all waves absent. In 3 cases of AN with other peripheral neuropathy which ABR were both ears 1 or 2 small wave ear recorded. However, 1 patient in our sample could not be detected distortion product otoacoustic emission at 3-6 kHz (left ear) and 5-6 kHz (right ear).</p><p><b>CONCLUSIONS</b>AN was not rare in adolescence. The average hearing threshold for AN should be discussed.</p>