Biodegradation of tetrachlorothylene using methanol as co-metabolic substrate / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate the biodegradation of tetrachloroethylene (PCE) using methanol as electron donor by acclimated anaerobic sludge.</p><p><b>METHODS</b>HP-6890 gas chromatograph (GC), together with HP-7694 autosampler, was used to analyze the concentration of PCE and intermediates.</p><p><b>RESULTS</b>PCE could be decholrinated reductively to DCE via TCE, and probably further to VC and ethylene. The degradation of PCE and TCE conformed to first-order reaction kinetics. The reaction rate constants were 0.8991 d(-1) and 0.068 d(-1), respectively, and the corresponding half-life were 0.77 d and 10.19 d, respectively. TCE production rate constant was 0.1333 d(-1), showing that PCE was degraded more rapidly than TCE.</p><p><b>CONCLUSION</b>Methanol is an electron donor suitable for PCE degradation and the cometabolic electron donors are not limiting factors for PCE degradation.</p>

Anaerobic degradation of tetrachloroethylene using different co-substrates as electron donors / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate the biodegradation of tetrachloroethylene (PCE) by acclimated anaerobic sludge using different co-substrates, i.e., glucose, acetate, and lactate as electron donors.</p><p><b>METHODS</b>HP-6890 gas chromatograph (GC) in combination with auto-sampler was used to analyze the concentration of PCE and its intermediates. Results PCE could be degraded by reductive dechlorination and the degradation reaction conformed to the first-order kinetic equation. The rate constants are k(lactate) > k(glucose) > k(acetate). The PCE degradation rate was the highest in the presence of lactate as an electron donor.</p><p><b>CONCLUSION</b>Lactate is the most suitable electron donor for PCE degradation and the electron donors supplied by co-metabolic substrates are not the limiting factors for PCE degradation.</p>