ABSTRACT
Objective To obtain comprehensive expression profile of microRNAs(miRNAs)in endometrial cancer stem cells(ECSCs)during differentiation.Methods ECSCs were enriched by spheroid formation and spontaneously differentiated into cancer cells by attached culture.We characterized miRNA expression in ECSCs and its differentiated cells using miRNA microarrays and verified the results by RT-qPCR.Meanwhile,we applied bioinformatic method to predict the target genes of the differentially expressed miRNAs.Furthermore,gene expression profile was detected by genomic microarray.Finally,we summarized the relationship between the predicted target genes of miRNAs and gene expression profile.Results We observed that 10 miRNAs were significantly upregulated in ECSCs,including miR-522,miR-139-3p,miR-520c-5p,miR-518d-5p,miR-146b-5p,miR-34a,miR-526a,miR-193a-3p,miR-221,and miR-4674.Only 1 miRNA,miR-760,was downregulated in ECSCs.A total of 11 differentially expressed miRNAs were identified by RT-qPCR.The results showed these differentially expressed miRNAs(except miR-526a and miR-4674)were in accordance with those obtained by miRNA microarray analysis. By using bioinformatic approach,the target genes of these differentially expressed miRNAs could be found.The results of genomic microarray showed that 207 genes were more highly expressed and 238 genes were more lowly expressed in ECSCs compared with its differentiated cells.Analysis of the gene expression profile and the predicted target genes of miRNAs showed that some common genes except miR-139-3p could be found.Conclusion Our study provides an available information about miRNAs and target genes from different starting points,such as ECSCs differentiation.Further studies are needed to ascertain the role of these miRNAs in ECSCs'differentiation.
ABSTRACT
In this article, medication characteristics of professor Li Dian-gui in treating chronic atrophic gastritis with intestinal metaplasia(CAGIM) were analyzed through traditional Chinese medicine inheritance support system(version 2.5). 276 cases and 625 prescriptions were collected to analyze five types of traditional Chinese medicine(TCM) syndromes and the medicine-syndrome correlation. The results showed that medication characteristics of professor Li Dian-gui in treating CAGIM included drug combination of aromatic medicine bitter-cold herbs, preferring to activating to invigorate the spleen and good at using the qi-regulating drugs. It demonstrated that we can adopt the therapy of Huazhuo Jiedu and Xingpi Xingqi therapies in treating CAGIM in addition to the traditional approach of nourishing Yin and activating blood circulation, opening up a novel approach for TCM in healing the pathema.