Research Progress on the Detection Method of DNA Methylation and Its Application in Forensic Science / 法医学杂志

As an important part of epigenetic marker, DNA methylation involves in the gene regulation and attracts a wide spread attention in biological auxology, geratology and oncology fields. In forensic science, because of the relative stable, heritable, abundant, and age-related characteristics, DNA methylation is considered to be a useful complement to the classic genetic markers for age-prediction, tissue-identification, and monozygotic twins' discrimination. Various methods for DNA methylation detection have been validated based on methylation sensitive restriction endonuclease, bisulfite modification and methylation-CpG binding protein. In recent years, it is reported that the third generation sequencing method can be used to detect DNA methylation. This paper aims to make a review on the detection method of DNA methylation and its applications in forensic science.

Effects of moxibustion on erectile function and NO-cGMP pathway in diabetic rats with erectile dysfunction / 中国针灸

<p><b>OBJECTIVE</b>To explore the effect and the mechanism of moxibustion for treatment of diabetic erectile dysfunction (ED).</p><p><b>METHODS</b>Diabetes mellitus (DM) rat model was induced by streptozotozin (STZ) and then penis erectile experiment of apomorphine (APO) was used to select diabetic ED rats model, which were divided into 2 groups: a model group and a moxibustion group, with another normal control group set up. The moxibustion group were treated with moxibustion at "Shenshu" (BL 23) and "Sanyinjiao" (SP 6) with small moxa cone about the size of a wheat grain. The effects on penis erectile, blood sugar and total NOS, cNOS, iNOS, and cGMP were investigated.</p><p><b>RESULTS</b>Moxibustion had a certain improving action on blood sugar, improved significantly erectile function, and more significantly increased NOS, cNOS, iNOS activities and cGMP contents in the penis.</p><p><b>CONCLUSION</b>Moxibustion has a certain action on the erectile function in the diabetic ED rats, which is related with improvement of blood sugar and promoting NO-cGMP pathway.</p>