Protective effect of carnosine on the injury of rat vascular endothelial cells induced by hypoxia / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To investigate the effect of camosine on the injury of rat vascular endothelial cells(VECs) induced by hypoxia.</p><p><b>METHODS</b>The model of the injury of rat VECs induced by hypoxia was established. The effect of camosine on injury of VECs activity induced by hypoxia was determined by MTT assay. The levels of lactate dehydrogenase (LDH) activity in cell medium were measured with corresponding kit. The cell structure was observed under microscope after Coomassie brilliant blue R-250 staining.</p><p><b>RESULTS</b>After culturing VECs with camosine (10 to 20 mmol/L) for 6 hours, the decrease in VECs activity induced by 12 and 24 hour hypoxia was inhibited. The release of LDH was also inhibited, and the integrity of cell structure remained.</p><p><b>CONCLUSION</b>Camosine has the protective effect on hypoxia injured VECs.</p>

Progress in individual identification of burned bones / 法医学杂志

The burned bone DNA test have became more and more important in identifying the individuals and paternity involved in the fire, explosion disasters as well as burn corpse crimes. As an important genetic marker system, STR has been widely used in forensic individual identification, paternity test and other fields. In this article, the influence of burned temperature and time to STR typing was reviewed, the choice of STR locus and DNA extraction methods were discussed about burned bones.