ABSTRACT
Objective: To investigate the effect of Yangzheng Sanjie decoction on proliferation, apoptosis and extracellular signal-regulated kinase (ERK) pathway of human gastric cancer MKN-45 cells.Method: Gastric cancer cell line MKN-45 was treated for 24, 48, 72 h with Yangzheng Sanjie decoction (0.5, 1, 1.5, 2, 2.5, 3, 3.5 g·L-1); cell proliferation was measured by cell counting kit-8 (CCK-8); cell colony forming ability was observed by the plate cloning experiment after intervention with Yangzheng Sanjie decoction (0.4, 0.8 g·L-1); MKN-45 cells was treated with 4, 8 g·L-1, and then cell apoptosis was detected by flow cytometry; the expression of ERK and its phosphorylation level were detected by Western blot assay after treatment with 2, 4, 8 g·L-1.Result: Compared with the blank group, Yangzheng Sanjie decoction could significantly inhibit the proliferation of MKN-45 cells. After treatment for 24, 48 h, Yangzheng Sanjie decoction started from 2 g·L-1, and after treatment for 72 h, it started from 1.5 g·L-1, the cell viability gradually decreased in a concentration-dependent manner (PPP-1, cell colonies could not be formed; the apoptosis rate of Yangzheng Sanjie decoction was significantly higher than that of the blank group (PP-1, and the phosphorylation level of ERK protein in MKN-45 cells was down-regulated (PConclusion: Yangzheng Sanjie decoction can inhibit the proliferation of human gastric cancer cell line MKN-45 and promote its apoptosis. The mechanism may be related to the inhibition of phosphorylation of ERK.
ABSTRACT
<p><b>OBJECTIVE</b>The purpose of this study was to investigate the clinical significance of THY1 protein expression in epithelial ovarian cancer.</p><p><b>METHODS</b>Immunohistochemistry (IHC) and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining were used to detect the protein expression of THY1, Ki67 and cell apoptosis in 76 epithelial ovarian cancers by tissue microarray. The correlation between THY1 expression and patients' clinical features was analyzed.</p><p><b>RESULTS</b>Of the 76 epithelial ovarian cancer samples, 64 were informative for IHC and TUNEL assays and 42 (65.6%) among them showed down-regulated/loss expression of THY1 protein. A significant positive correlation of THY1 protein expression with clinical stage and distant metastasis was observed in this ovarian cancer cohort (P < 0.05). The more advanced the tumor stage, the more frequency of loss expression of THY1 protein. In addition, the mean positive rate of Ki67 staining in tumors with down-regulated/loss expression of THY1 was 33.7% +/- 3.5%, significantly higher than that in the tumors with normal expression of THY1 (17.3% +/- 6.1%, P = 0.0027). However, no significant correlation was observed between THY1 protein expression and tumor cell apoptosis as well as patients' survival in this series (P > 0.05).</p><p><b>CONCLUSION</b>Down-regulated/loss expression of THY1 protein in epithelial ovarian cancer is significantly correlated with cancer cell proliferation and metastasis in the epithelial ovarian cancer, and it may be used as one of the new molecular biomarkers to predict the disease progression in patients.</p>
Subject(s)
Adult , Aged , Female , Humans , Middle Aged , Apoptosis , Cystadenocarcinoma, Mucinous , Metabolism , Pathology , Cystadenocarcinoma, Serous , Metabolism , Pathology , Down-Regulation , Follow-Up Studies , Gene Expression Regulation, Neoplastic , Ki-67 Antigen , Metabolism , Neoplasm Metastasis , Neoplasm Staging , Ovarian Neoplasms , Metabolism , Pathology , Survival Rate , Thy-1 Antigens , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To investigate the therapeutic mechanism of Jiawei Mojie Tablet (JMT) in treating primary dysmenorrhea (PD) by observing its effect on plasma oxytocin (OT) level in patients in menstrual period.</p><p><b>METHODS</b>Sixty-three patients with PD enrolled in the study were randomly divided into the JMT group (n = 33) and the control Group (n = 30, treated with Yueyueshu Granule) to observe the change of OT level in the menstrual period before and after treatment.</p><p><b>RESULTS</b>OT level in patients with PD was obviously higher than that in healthy subjects (P < 0.01), and was positively correlated with the degree of pain (r = 0.738, P < 0.01). OT level reduced significantly after treatment (P < 0.01), the reduction was more significant in the JMT group than that in the control group (P < 0.01).</p><p><b>CONCLUSION</b>JMT could reduce the OT level in menstrual period.</p>