ABSTRACT
This study was to investigate the role of stromal cell-derived factor 1 [SDF-1] and its corresponding receptor CXCR4 in the proliferation, migration and invasion of cervical cancer HeLa cells. CXCR4 expression in HeLa cells was measured by flow cytometry and Western Blot. Role of SDF-1 and CXCR4 in the HeLa cells proliferation was measured by MTT. Role of SDF-1 and CXCR4 in the migration and invasion of HeLa cell was measured by Boyden chamber. High expression of CXCR4 was observed on the surface of HeLa cells. Proliferation ability of HeLa cells was significantly increased after SDF-1 stimulation, which showed dose-dependent manner. After knock-down of CXCR4 expression by RNAi, SDF-1-stimulated HeLa cells proliferation was significantly blocked [P<0.05]. SDF-1 can induce migration and invasion of Hela cells, SDF-1-stimulated HeLa cells migration and invasion was significantly blocked [P<0.05] after knock-down of CXCR4 expression by RNAi. High expression of surface CXCR4 plays an important role in the proliferation, migration and invasion of HeLa cells
ABSTRACT
To explore the correlation between magnetic resonance imaging [MRI] features of uterine fibroids [UFs] and therapeutic effects of high-intensity focused ultrasound ablation [HIFUA], and to provide evidence for UFs diagnosis with MRI in clinical practice. Forty-three UFs patients who were treated in our hospital from April 2012 to June 2014 were selected, including 72 UFs [48 multiple and 24 single UFs]. Transverse, sagittal and coronal MRI scanning was performed one week before and after HIFUA to record UF number, location, type [intramural fibroid, submucosal fibroid and subserosal fibroid], mean diameter, hemoperfusion state, volume and ablation rate. The patients were followed up in the postoperative 1st, 2nd and 3rd months. HIFUA exerted the best ablative effect on fibroids on the anterior uterine wall [F=26.763, P=0.036]. Various types of fibroids were ablated significantly differently [F=3.406, P<0.05] by HIFUA that was most effective for ablating the subserosal ones. Having significantly different ablative effects on UFs with different radial line lengths [F=29.94, P<0.05], HIFUA ablated those with radial line lengths of 3-5 cm most effectively. For UFs with different T2WI signal intensities, HIFUA also functioned significantly differently [F=3.179, P=0. 03]. HIFUA exerted significantly different ablative effects on UFs with various MRI features. Therefore, these features were well correlated with the therapeutic effects of HIFUA, allowing MRI as a promising diagnostic protocol