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1.
China Journal of Chinese Materia Medica ; (24): 2666-2668, 2010.
Article in Chinese | WPRIM | ID: wpr-279379

ABSTRACT

<p><b>OBJECTIVE</b>To compare the rutin and syringin content in tissue culturing seedlings and in botanical drug of Saussurea involucrata.</p><p><b>METHOD</b>The HPLC with Hydro-RP C18 (4.6 mm x 250 mm, 5 microm) column was used, a mixture of acetonitrile-water (5:95) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 25 degrees C and detection wavelength at 220 nm.</p><p><b>RESULT</b>The effective constituents of tissue culturing seedlings were almost similar to the botanical drug. And syringin in tissue culturing seedlings was increased 4.35 times.</p><p><b>CONCLUSION</b>It has a good prospect to acquire high-quality S. involucrata by tissue culturing seedlings.</p>


Subject(s)
Chromatography, High Pressure Liquid , Glucosides , Phenylpropionates , Rutin , Saussurea , Chemistry , Seedlings , Chemistry
2.
China Journal of Chinese Materia Medica ; (24): 1715-1719, 2010.
Article in Chinese | WPRIM | ID: wpr-328074

ABSTRACT

The column chromatography on silica gel, sephadex LH-20 preparative HPLC were used to separate and purify the compounds from the stems of Dendrobium candidum. Twenty compounds were isolated and identified as 3,4'-dihydroxy-5-methoxybibenzyl(1), dihydroresveratrol(2), dendromoniliside E(3), denbinobin(4),2,4,7-trihydroxy-9, 10-dihydrophenanthrene(5), aduncin(6), (-)-loliolide(7), adenosine(8), uridine(9), guanosine(10), sucrose(11), 5-hydroxymethyl-furaldehyde(12), n-octacostyl ferulate(13), defuscin(14), n-triacontyl cis-p-coumarate(15), daucosterol(16), beta-sitosterol(17), hexadecanoic acid(18), hentriacontane(19), and heptadecanoic acid(20). Their structures were elucidated on the basis of spectroscopic data and physicochemical properties. All of the compounds were isolated from this plant for the first time.


Subject(s)
Dendrobium , Chemistry , Molecular Structure , Plant Extracts , Chemistry
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