ABSTRACT
In vitro differentiation of bone marrow stromal stem cells (BMSCs) into neural cells has been a hot study in neuroscience. Presently, under some experimental conditions, tissue-specific stem cells have been shown to give rise to cell lineages not normally found in the organ or tissue of residence. BMSCs have been reported to generate skeletal cells, gastric cardiac cells, oval hepatocytes, as well as glia and neuron-like cells. Cells from postnatal bone marrow were induced to proliferate and differentiate into gila and neurons. The present study explored the characteristics of its transformation.
ABSTRACT
AIM:To evaluate the effect of basic fibroblast growth factor(bFGF)on the expression of cyclin D1,growth arrest,DNA damage inducible gene 153(GADD153),and its roles involved in cell cycle regulation and DNA repair in starvation-induced ovarian cancer CAOV3 cells apoptosis.METHODS:Apoptosis of ovarian cancer CAOV3 cells was induced by serum-free culture(starvation).After bFGF treatment,the cell proliferation rate,cell cycle and apoptosis were determined by MTT,FACS analysis and agarose electrophoresis,respectively.The expression of c-Fos,c-Jun and cyclin D1,GADD153 were detected by Western blotting.RESULTS:bFGF increased the cell proliferation and prevented starvation-induced cell apoptosis.In a time-dependent manner,bFGF induced the expression of c-Fos,c-Jun and cyclin D1 and inhibited GADD153.CONCLUSION:bFGF plays a critical role in anti-apoptosis and the proliferation in human ovarian cancer by upregulating the expression of c-Fos,c-Jun and cyclin D1 and inhibiting GADD153.