ABSTRACT
Objective:To investigate the relationship of lipid peroxide (LPO), total oxidation state (TOS), apolipoprotein (a) [apolipoprotein(a), Apo(a)] and pregnancy outcome in patients with polycystic ovarian syndrome (PCOS) treated by ovulation induction-in vitro fertilization-embryo transfer (IVF-ET).Methods:The clinical of 215 patients with PCOS treated by IVF-ET who were admitted to Hengshui People′s Hospitalfrom May 2017 to February 2020 were collected and they were divided into clinical pregnancy group (155 cases) and biochemical pregnancy group (60 cases) according to pregnancy outcome. The levels of LPO, TOS, and Apo(a) in the peripheral blood of the two groups were detected and the data were analyzed.Results:The levels of LPO and TOS before ovulation induction and human chorionic gonadotropin (HCG) dayin the biochemical pregnancy group were higher than those in the clinical pregnancy group: (10.35 ± 3.67) μmol/L vs. (7.16 ± 1.59) μmol/L, (17.98 ± 3.15) mmol H 2O 2 equiv/L vs. (15.03 ± 3.21) mmol H 2O 2 equiv/L; (12.81 ± 4.09) μmol/L vs. (7.38 ± 2.14) μmol/L, (19.66 ± 3.02) mmol H 2O 2 equiv/L vs. (15.19 ± 3.34) mmol H 2O 2 equiv/L; and the level of Apo(a) was lower than that in the clinical pregnancy group: (379.8 ± 95.9) mg/L vs. (486.5 ± 100.3) mg/L, (335.8 ± 84.7) mg/L vs. (473.5 ± 112.9) mg/L, the differences were statistically significant ( P<0.05). LPO and TOS before ovulation induction and HCG day were negatively correlated with the number of high-quality embryos ( P<0.01), and Apo(a) was positively correlated with the number of high-quality embryos ( P<0.01). The risk of non-clinical pregnancy for those with LPO, TOS, Apo(a) higher than the average before ovulation induction was 1.435, 1.233, 0.678 times of those with lower than the average ( P<0.05). The risk of non-clinical pregnancy for those with LPO, TOS, and Apo(a) higher than the average on HCG day was 1.443, 1.689, 0.762 times of those with lower than average ( P<0.05). After receiver operating characteristic (ROC) curve analysis, the area under the curve(AUC) of all indicators before ovulation induction combined to predict clinical pregnancy was 0.844. The AUC of all indicators on HCG day combined to predict clinical pregnancy was 0.894. Conclusions:Peripheral blood LPO, TOS, Apo(a) levels are closely related to the number of high-quality embryos, and are the main influencing factors of pregnancy outcome. Therefore, dynamic monitoring of the above-mentioned index levels can provide a reference for the clinical improvement of the treatment plan.