ABSTRACT
Objective To evaluate the role of myricetin in the metastasis of human renal cancer ACHN cells both in vitro and in vivo.Methods With different concentration of myricetin (25, 50, 100 μmol/L) acting on ACHN cells, the cell viability, migration and invasion were respectively measured with CCK-8 assay, wound healing migration assay and Transwell invasion assay in vitro.A lung metastatic model for human renal cell carcinoma was developed by tail vein injection with ACHN cells.The effect of myricetin on the metastasis of renal cell carcinoma was explored by intraperitoneal injection of myricetin in vivo.After 6 weeks, the micro metastases of the lungs in nude mice was examined.Results Compared to the control group, CCK-8 assay showed that the cell viability was significantly lower in the myricetin group at the concentration of 50 μmol/L for 24 h (P=0.019).The inhibitory effect became more significant with the increase in drug concentration or action time.In addition, myricetin also inhibits the migration and invasion of ACHN cells in a dose dependent manner.Intraperitoneal injection of myricetin significantly reduced the number of the micro metastases of renal cell carcinoma in the lungs of nude mice.Conclusion Myricetin effectively inhibits the cell viability, migration and invasion of human renal cancer ACHN cells.It has the potential therapeutic value in the treatment of renal cell carcinoma.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility of constructing a capsular poly L-lactic acid (PLLA) ureteral stent seeded with autologous urothelial cells using tissue engineering methods.</p><p><b>METHODS</b>The capsular ureteral stent was constructed by subcutaneously embedding PLLA ureteral stent in the back of beagles for 3 weeks to induce the formation of connective tissue on the surfaces. After decellularization of the stent, the expanded autologous urothelial cells were seeded on the stent. The surface structure and cell adhesion of the stent were observed using HE staining, scanning electron microscope (SEM) and immunocytochemical staining. MTT assay was used to evaluate urothelial cell proliferation on the capsular PLLA ureteral stent and on circumferential small intestinal submucosa graft.</p><p><b>RESULTS</b>HE staining and VIII factor immunohistochemistry revealed numerous capillaries in the connective tissue encapsulating the stent without obvious local inflammatory response. The results of SEM and immunocytochemical staining showed that the capsule contained rich collagenic fibers forming three-dimensional structures, and the seeded autologous urothelial cells could adhere and well aligned on the surface. MTT assay showed normal growth of the cells on the stent as compared with the cells grown on circumferential small intestinal submucosa graft.</p><p><b>CONCLUSION</b>The capsular PLLA ureteral stent allows adhesion and proliferation of autologous urothelial cells and shows a potential in applications of constructing tissue-engineered ureter.</p>
Subject(s)
Animals , Dogs , Female , Bacterial Adhesion , Cell Proliferation , Epithelial Cells , Cell Biology , Transplantation , Lactic Acid , Polyesters , Polymers , Stents , Tissue Engineering , Methods , Transplantation, Autologous , Ureter , General Surgery , Urothelium , Cell BiologyABSTRACT
Objective To evaluate effects of three modalities with intravenous immrnoglobulin(IVIG) infusion in the treatment of Kawasaki disease(KD). Methods 76 KD cases were treated by the IVIG in the Deparment of Pediatric of Kaiping Central Hospital from 1998 to 2004.The three modalities were as follows: four days treatment(0.4 g/kg,for four days continuance),two days treatment(1 g/kg,for two days continuance),one day treatment(2 g/kg,for one day).Results(1) In treatment of fever,conjunctiva congest and mouth change,and recover in white blood cell and erythrocyte sedimentation,one day treatment and two days treatment had better curative effects with significantly difference(P0.05).(3)In the prevention of coronary artery dilate(CAD),one day treatment and two days treatment had better curative effects significantly difference(P