ABSTRACT
Objective To study the expressions of YKL-40 and IL-1β in the cartilage of knee osteoarthritis(KOA),and analysis the possible relationship between YKL-40,IL-1β in KOA.Methods Thirty-eight patients diagnosed with KOA were selected in this hospital as observation group.Then select 30 articular cartilage cases in the same period due to knee injury knee joint examina tion or treatment of knee joint fracture surgery patients,referred to as the control group.According to KOA radiation and arthroscopic grading standards,the observation group was divided into 16 cases of mild group,10 cases of moderate group,11 cases of severe groups.Compare the expression levels of all patients including YKL-40,interleukin-1β (IL-1β),IL 6,tumor necrosis factor-α (TNF-α),while recording Mankin score and cell mortality.Results Expression of YKL 40,IL-1β,IL-6,TNFα,Mankin scores and rate of cell death about observation group were significantly higher than control group,the indicators above in mild,moderate,severe group showed a trend of rising(all P＜0.05).And the expression levels of YKL-40 and IL-1β were significantly positive correlation (r=0.738,P=0.000).In addition, The expression levels of YKL-40 had relation with IL 6 (r=0.819,P=0.000),TNF-α (r=0.871,P=0.000) and Mankin score (r=0.832,P 0.000),cells mortality deposit (r=0.832,P=0.000).Conclusion Expression levels of YKL-40 and IL-1β were significantly increased in cartilage of knee osteoarthritis patients,and there showed a significant positive correlation between YKL-40 and IL-1β.
ABSTRACT
BACKGROUND:Spinal surgery requires a relatively high accuracy. At present, the study of pedicle screw fixation is more focused on the safety and stability, but the premise is the safe and effective screw placement. Selection of the insertion point is the key to success. OBJECTIVE:To analyze stress distribution of different pedicle screw implantation methods for thoracolumbar fractures by using finite element analysis. METHODS:T9-L3 segments of a thoracolumbar burst fractures patient were scanned in the Department of Orthopedics, Zhaoqing City First People’s Hospital in January 2015. 616 CT Dicom images were obtained and introduced into engineering software. Finite element geometric model was established. Posterior approach orthopedic surgery was simulated. Stress distribution after pedicle screw fixation for thoracolumbar fractures was analyzed. RESULTS AND CONCLUSION:(1) The three-dimensional motion range of the fixation with six screws and two rods was small in anteflexion, lateral bending, and axial rotation, followed by five screws and two rods by posterior approach (single right vertebral fixation). The motion range of the fixation with four screws and two rods was biggest, but biomechanical stability was poorest. (2) In different fixation models, upper screw stress was larger in anteflexion than other states in the fixation with four screws and two rods by posterior trans-traumatic-vertebra, four screws and two rods by posterior injured vertebra, six screws and two rods by posterior injured vertebra, five screws and two rods by posterior approach (single right injured vertebra) and six screws and two rods by posterior approach (upper injured vertebra), followed by left axial rotation and right axial rotation. Significant differences in screw stress were found in different motion states (P < 0.05). (3) In different fixation modes, the stress ratio of upper and lower screws was significantly larger in the fixation with four screws and two rods by posterior trans-traumatic-vertebra, four screws and two rods by posterior injured vertebra, six screws and two rods by posterior injured vertebra, five screws and two rods by posterior approach (single right injured vertebra) and six screws and two rods by posterior approach (upper injured vertebra) than other motion states (P < 0.05), followed by right axial rotation and right lateral bending. Significant differences in stress ratio of upper and lower screws were detectable in different motion states (P < 0.05). (4) Results suggested that finite element analysis could better simulate complex spine mechanical system, accurately reflect the stress distribution of spinal pedicle screw model, and provide the basis for making pedicle screw program.